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TBL2 Monoclonal antibody

TBL2 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgA

Reactivity

human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1D5C2

Cat No : 66307-1-Ig

Print datasheet

Synonyms

TBL2, transducin (beta) like 2, Transducin beta like protein 2, WBSCR13, WS betaTRP

验证数据展示

  • WB analysis of MCF-7 using 66307-1-Ig

    WB analysis of MCF-7 using 66307-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66307-1-Ig (TBL2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 66307-1-Ig

    IHC staining of human liver using 66307-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66307-1-Ig (TBL2 Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inMCF-7 cells, COLO 320 cells, Jurkat cells
Positive IHC detected inhuman liver tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Mouse monoclonal antibodies of IgA isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66307-1-Ig targets TBL2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human
Immunogen TBL2 fusion protein Ag24225 种属同源性预测
Host / Isotype Mouse / IgA
Class Monoclonal
Type Antibody
Full Name transducin (beta)-like 2
Synonyms TBL2, transducin (beta) like 2, Transducin beta like protein 2, WBSCR13, WS betaTRP
Calculated Molecular Weight 446 aa, 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC012938
Gene Symbol TBL2
Gene ID (NCBI) 26608
RRIDAB_2881689
Conjugate Unconjugated
Form Liquid
Purification MethodThiophilic affinity chromatograph
UNIPROT IDQ9Y4P3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TBL2 belongs to a member of the beta-transducin protein family. This gene is commonly deleted in Williams-Beuren syndrome, a developmental disorder with multi-system manifestations caused by haploinsufficiency for contiguous genes deleted in chromosome region 7q11.23. TBL2 encodes a protein with four putative WD40 repeats, which may be involved in some intracellular signaling pathway.

实验方案

Product Specific Protocols
WB protocol for TBL2 antibody 66307-1-IgDownload protocol
IHC protocol for TBL2 antibody 66307-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols