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CoraLite® Plus 488-conjugated TBCB Polyclonal antibody

TBCB Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-15782

Print datasheet

Synonyms

CG22, CKAP1, CKAPI, TBCB, tubulin folding cofactor B, Tubulin specific chaperone B

验证数据展示

  • FC experiment of HeLa using CL488-15782

    FC experiment of HeLa using CL488-15782

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 TBCB Polyclonal Antibody (CL488-15782)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 15782-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-15782 targets TBCB in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra)
Tested Reactivity human, mouse, rat
Immunogen TBCB fusion protein Ag8473 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tubulin folding cofactor B
Synonyms CG22, CKAP1, CKAPI, TBCB, tubulin folding cofactor B, Tubulin specific chaperone B
Calculated Molecular Weight 244 aa, 27 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC005969
Gene Symbol TBCB
Gene ID (NCBI) 1155
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99426
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 TBCB antibody CL488-15782Download protocol
Standard Protocols
Click here to view our Standard Protocols