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验证数据展示

  • WB analysis using 24462-1-AP

    WB analysis using 24462-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24462-1-AP (TBC1D2B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of U-251 using 24462-1-AP

    WB analysis of U-251 using 24462-1-AP

    WB result of TBC1D2B antibody (24462-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TBC1D2B transfected U-251 cells.

  • IP experiment of BxPC-3 using 24462-1-AP

    IP experiment of BxPC-3 using 24462-1-AP

    IP result of anti-TBC1D2B (IP:24462-1-AP, 4ug; Detection:24462-1-AP 1:600) with BxPC-3 cells lysate 1200 ug.

  • KD/KO Validated

TBC1D2B Polyclonal antibody

TBC1D2B Polyclonal Antibody for WB, IP, ELISA
Cat No. 24462-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, ELISA

别名

TBC1 domain family member 2B, TBC1 domain family, member 2B

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inBxPC-3 cells, U-251 cells, HL-60 cells, mouse spleen tissue
Positive IP detected inBxPC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24462-1-AP targets TBC1D2B in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag19632

Product name: Recombinant human TBC1D2B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 324-655 aa of BC152465

Sequence: KDEVIIKLSEGEGNGPPPTVAPSSPSVVPVARDQLELDRLKDNLQGYKTQNKFLNKEILELSALRRNAERRERDLMAKYSSLEAKLCQIESKYLILLQEMKTPVCSEDQGPTREVIAQLLEDALQVESQEQPEQAFVKPHLVSEYDIYGFRTVPEDDEEEKLVAKVRALDLKTLYLTENQEVSTGVKWENYFASTVNREMMCSPELKNLIRAGIPHEHRSKVWKWCVDRHTRKFKDNTEPGHFQTLLQKALEKQNPASKQIELDLLRTLPNNKHYSCPTSEGIQKLRNVLLAFSWRNPDIGYCQGLNRLVAVALLYLEQEDAFWCLVTIVEV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TBC1 domain family, member 2B
别名 TBC1 domain family member 2B, TBC1 domain family, member 2B
计算分子量 963 aa, 110 kDa
观测分子量 120-125 kDa
GenBank蛋白编号BC152465
基因名称 TBC1D2B
Gene ID (NCBI) 23102
RRIDAB_3742115
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UPU7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TBC1 domain family member 2B (TBC1D2B) contains a functional canonical LIR motif and acts at an early stage of autophagy by binding to both LC3/GABARAP and ATG12 conjugation complexes (PMID: 38226533). When there is a high protein level of RAB22A on early endosome, TBC1D2B is recruited and acts as a brake to prevent early endosomes from maturing into late endosomes (PMID: 39051763).

实验方案

Product Specific Protocols
IP protocol for TBC1D2B antibody 24462-1-APDownload protocol
WB protocol for TBC1D2B antibody 24462-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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