验证数据展示

WB analysis of HEK-293 using 17002-1-AP
WB result of TBC1D23 antibody (17002-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TBC1D23 transfected HEK-293 cells.
WB analysis using 17002-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17002-1-AP (TBC1D23 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IF Staining of U2OS using 17002-1-AP
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using TBC1D23 antibody (17002-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
IP experiment of HepG2 using 17002-1-AP
IP result of anti-TBC1D23 (IP:17002-1-AP, 4ug; Detection:17002-1-AP 1:500) with HepG2 cells lysate 2400ug.
IF Staining of HeLa using 17002-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TBC1D23 antibody (17002-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
WB analysis of HepG2 using 17002-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 17002-1-AP (TBC1D23 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human stomach cancer using 17002-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17002-1-AP (TBC1D23 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with TBC1D23 antibody included
IHC staining of rat liver using 17002-1-AP
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 17002-1-AP (TBC1D23 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with TBC1D23 antibody included
IHC staining of rat stomach using 17002-1-AP
Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 17002-1-AP (TBC1D23 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with TBC1D23 antibody included

Featured Product
KD/KO Validated

TBC1D23 Polyclonal antibody

TBC1D23 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 17002-1-AP

产品说明书

引用文献(12)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

HCV non-structural protein 4A-transactivated protein 1, NS4ATP1, TBC1 domain family member 23, TBC1 domain family, member 23

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (12)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HEK-293T cells, HEK-293 cells, HeLa cells, HepG2 cells
Positive IP detected in	HepG2 cells
Positive IHC detected in	human stomach cancer tissue, human spleen tissue, human kidney tissue, human ovary tissue, human testis tissue, human liver tissue, human placenta tissue, rat liver tissue, mouse ovary tissue, rat ovary tissue, rat stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	U2OS cells, HeLa cells

Planning an IHC experiment? We recommend our IHCeasy TBC1D23 Ready-To-Use IHC Kit. TBC1D23 primary antibody included.

应用	推荐稀释比
Western Blot (WB)	WB : 1:600-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 4 publications below
WB	See 10 publications below
IHC	See 2 publications below
IF	See 4 publications below
IP	See 2 publications below

产品信息

17002-1-AP targets TBC1D23 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse
免疫原	CatNo: Ag10697 Product name: Recombinant human TBC1D23 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-320 aa of BC010221 Sequence: MLQNPSEFAQSVKSLLEAQKQSIESGSIAGGEHLCFMGSGREEEDMYMNMVLAHFLQKNKEYVSIASGGFMALQQHLADINVDGPENGYGHWIASTSGSRSSINSVDGESPNGSSDRGMKSLVNKMTVALKTKSVNVREKVISFIENTSTPVDRHVSSSDRVGKPYRGVKPVFSIGDEEEYDTDEIDSSSMSDDDRKEVVNIQTWINKPDVKHHFPCKEVKESGHMFPSHLLVTATHMYCLREIVSRKGLAYIQSRQALNSVVKITSKKKHPELITFKYGNSSASGIEILAIERYLIPNAGDATKAIKQQIMKVLDALES 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	TBC1 domain family, member 23
别名	HCV non-structural protein 4A-transactivated protein 1, NS4ATP1, TBC1 domain family member 23, TBC1 domain family, member 23
计算分子量	699 aa, 78 kDa
观测分子量	70-78 kDa
GenBank蛋白编号	BC010221
基因名称	TBC1D23
Gene ID (NCBI)	55773
RRID	AB_2240206
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	Q9NUY8
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

TBC1D23, also named as NS4ATP1, is a gene down-regulated by Pneumocystis infection. Pneumocystis pneumonia is a common opportunistic disease in AIDS patients. (PMID: 20377877) Recently it has been found that TBC1D23 gene is the most frequently mutated in MSI-H cell lines and protein expressions of TBC1D23 in tumors with mutation were down regulated.(PMID: 20824714) This antibody is specific to TBC1D23.

实验方案

Product Specific Protocols
IF protocol for TBC1D23 antibody 17002-1-AP	Download protocol
IHC protocol for TBC1D23 antibody 17002-1-AP	Download protocol
IP protocol for TBC1D23 antibody 17002-1-AP	Download protocol
WB protocol for TBC1D23 antibody 17002-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	Cell The WDR11 complex is a receptor for acidic-cluster-containing cargo proteins Authors - Huaqing Deng View Article
human	WB	Nat Commun A CRISPR/Cas9 screen reveals proteins at the endosome-Golgi interface that modulate cellular anti-sense oligonucleotide activity. Authors - Liza Malong KO Validated View Article
	WB	Nat Commun TBC1D23 mediates Golgi-specific LKB1 signaling Authors - Yingfeng Tu View Article
mouse,human	WB,IP	Nat Commun The functional landscape of alternative splicing in hematopoietic lineage commitment. Authors - Xiao Hu KO Validated View Article
mouse,human	WB	Cell Discov Trans-Golgi network tethering factors regulate TBK1 trafficking and promote the STING-IFN-I pathway Authors - Jinrui Wang KO Validated View Article
human	WB	Mol Cell Divergent proteome tolerance against gain and loss of chromosome arms. Authors - Yi Di View Article

验证数据展示

WB analysis of HEK-293 using 17002-1-AP

WB analysis using 17002-1-AP

IF Staining of U2OS using 17002-1-AP

IP experiment of HepG2 using 17002-1-AP

IF Staining of HeLa using 17002-1-AP

WB analysis of HepG2 using 17002-1-AP

IHC staining of human stomach cancer using 17002-1-AP

IHC staining of rat liver using 17002-1-AP

IHC staining of rat stomach using 17002-1-AP

TBC1D23 Polyclonal antibody

TBC1D23 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

The WDR11 complex is a receptor for acidic-cluster-containing cargo proteins

A CRISPR/Cas9 screen reveals proteins at the endosome-Golgi interface that modulate cellular anti-sense oligonucleotide activity.

TBC1D23 mediates Golgi-specific LKB1 signaling

The functional landscape of alternative splicing in hematopoietic lineage commitment.

Trans-Golgi network tethering factors regulate TBK1 trafficking and promote the STING-IFN-I pathway

Divergent proteome tolerance against gain and loss of chromosome arms.

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