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验证数据展示

  • WB analysis using 60975-3-Ig

    WB analysis using 60975-3-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60975-3-Ig (TBC1D17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 60975-3-Ig

    WB analysis using 60975-3-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60975-3-Ig (TBC1D17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

TBC1D17 Monoclonal antibody

TBC1D17 Monoclonal Antibody for WB, ELISA
Cat No. 60975-3-Ig

产品说明书

CloneNo. 1H3C1

宿主/亚型

Mouse / IgG2a

种属反应性

human, pig, rabbit

应用

WB, ELISA

别名

TBC1 domain family member 17, TBC1 domain family, member 17

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, pig brain tissue, LNCaP cells, HeLa cells, Jurkat cells, A172 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60975-3-Ig targets TBC1D17 in WB, ELISA applications and shows reactivity with human, pig, rabbit samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, pig, rabbit
免疫原

CatNo: Ag14358

Product name: Recombinant human TBC1D17 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC003516

Sequence: MEGAGYRVVFEKGGVYLHTSAKKYQDRDSLIAGVIRVVEKDNDVLLHWAPVEEAGDSTQILFSKKDSSGGDSCASEEEPTFDPGYEPDWAVISTVRPQPCHSEPTRGAEPSCPQGSWAFSVSLGELKSIRRSKPGLSWAYLVLVTQAGGSLPALHFHRGGTRALLRVLSRYLLLASSPQDSRLYLVFPHDSSALSNSFHHLQLFDQDSSNVVSRFLQDPYSTTFSSFSRVTNFFRGALQPQPEGAASDLPPPPDDEPEPGFEVISCVELGPRPTVERGPPVTEEEWARHVGPEGRLQQVPELKNRIFSGGLSPSLRREAWKFLLGYLSWEGTAEEHKAHIRKKTDEYFRM

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 TBC1 domain family, member 17
别名 TBC1 domain family member 17, TBC1 domain family, member 17
计算分子量 648 aa, 73 kDa
观测分子量 73 kDa
GenBank蛋白编号BC003516
基因名称 TBC1D17
Gene ID (NCBI) 79735
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9HA65
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TBC1 domain family member 17 (TBC1D17), a Rab GTPase-activating protein, through its catalytic activity, regulates Rab8-mediated endocytic trafficking of transferrin receptor (PMID: 22854040). TBC1D17 also inhibits the trafficking of Shiga toxin from plasma membrane to the Golgi complex through its catalytic activity (PMID: 1756278).

实验方案

Product Specific Protocols
WB protocol for TBC1D17 antibody 60975-3-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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