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TBC1D15 Polyclonal antibody

TBC1D15 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 17252-1-AP

Print datasheet

Synonyms

GAP for RAB7, GTPase activating protein RAB7, Rab7 GAP, TBC1 domain family member 15, TBC1 domain family, member 15, TBC1D15

验证数据展示

  • WB analysis using 17252-1-AP

    WB analysis using 17252-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17252-1-AP (TBC1D15 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis using 17252-1-AP

    WB analysis using 17252-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17252-1-AP (TBC1D15 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 17252-1-AP

    IF Staining of HeLa using 17252-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TBC1D15 antibody (17252-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HEK-293T cells, A431 cells, mouse liver tissue, rat liver tissue
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17252-1-AP targets TBC1D15 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen TBC1D15 fusion protein Ag10625 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TBC1 domain family, member 15
Synonyms GAP for RAB7, GTPase activating protein RAB7, Rab7 GAP, TBC1 domain family member 15, TBC1 domain family, member 15, TBC1D15
Calculated Molecular Weight 691 aa, 79 kDa
Observed Molecular Weight 79 kDa
GenBank Accession NumberBC028352
Gene Symbol TBC1D15
Gene ID (NCBI) 64786
RRIDAB_2878370
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TC07
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TBC1D15 is a highly conserved protein belonging to the TBC domain family and has similar functions to Rab-GAP. TBC1D15 is ubiquitously expressed and localized predominantly to the cytosol. TBC1D15 is a protein that contains a conserved Tre2/Bub2/Cdc16 (TBC) domain. TBC1D15 can serve as a mitochondrial Rab GTPase-activating protein (Rab-GAP) and influence autophagosome biogenesis and morphology downstream of Parkin activation. TBC1D15 can also control stem cell self-renewal when coupled with the Numb-p53 complex.

实验方案

Product Specific Protocols
WB protocol for TBC1D15 antibody 17252-1-APDownload protocol
IF protocol for TBC1D15 antibody 17252-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols