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验证数据展示

  • WB analysis using 82846-5-RR

    WB analysis using 82846-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82846-5-RR (TAU antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 82846-5-RR

    IHC staining of human colon using 82846-5-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82846-5-RR (TAU antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 82846-5-RR

    IHC staining of human colon using 82846-5-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82846-5-RR (TAU antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 82846-5-RR

    IF Staining of mouse brain using 82846-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TAU antibody (82846-5-RR, Clone: 252452C8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 82846-5-RR

    IF Staining of mouse brain using 82846-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TAU antibody (82846-5-RR, Clone: 252452C8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 82846-5-RR

    IF Staining of mouse brain using 82846-5-RR

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using TAU antibody (82846-5-RR, Clone: 252452C8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity of 82846-5-RR

    Affinity of 82846-5-RR

    Biolayer interferometry (BLI) kinetic assays of 82846-5-RR against Human TAU were performed. The affinity constant is below 1 pM.

TAU Recombinant monoclonal antibody

TAU Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, IF-Fro, ELISA
Cat No. 82846-5-RR

产品说明书

CloneNo. 252452C8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, IF-Fro, ELISA

别名

MAPT, AT180, DDPAC, MAPTL, Microtubule-associated protein tau

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue, fetal human brain tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF-Fro detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1500-1:6000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)-FROIF-FRO : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82846-5-RR targets TAU in WB, IHC, IF-P, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, IF-Fro, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Eg6232

Product name: Recombinant human TAU protein

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 1-758 aa of NM_016835.4

Sequence: MAEPRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDTDAGLKESPLQTPTEDGSEEPGSETSDAKSTPTAEDVTAPLVDEGAPGKQAAAQPHTEIPEGTTAEEAGIGDTPSLEDEAAGHVTQEPESGKVVQEGFLREPGPPGLSHQLMSGMPGAPLLPEGPREATRQPSGTGPEDTEGGRHAPELLKHQLLGDLHQEGPPLKGAGGKERPGSKEEVDEDRDVDESSPQDSPPSKASPAQDGRPPQTAAREATSIPGFPAEGAIPLPVDFLSKVSTEIPASEPDGPSVGRAKGQDAPLEFTFHVEITPNVQKEQAHSEEHLGRAAFPGAPGEGPEARGPSLGEDTKEADLPEPSEKQPAAAPRGKPVSRVPQLKARMVSKSKDGTGSDDKKAKTSTRSSAKTLKNRPCLSPKHPTPGSSDPLIQPSSPAVCPEPPSSPKYVSSVTSRTGSSGAKEMKLKGADGKTKIATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPKSGDRSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSSAKSRLQTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 microtubule-associated protein tau
别名 MAPT, AT180, DDPAC, MAPTL, Microtubule-associated protein tau
计算分子量79 kDa
观测分子量 45-70 kDa
GenBank蛋白编号NM_016835.4
基因名称 TAU
Gene ID (NCBI) 4137
RRIDAB_3743441
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP10636
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tau (tubulin-associated unit) is a microtubule-associated protein (also known as MAPT), expressed mainly in neurons of the central nervous system. Its primary function is to modulate microtubule dynamics for maintaining axonal cytoskeleton. Various isoforms of Tau exist due to the alternative splicing, and short isoforms around 45-69 kDa and long isoforms around 100-110 kDa have been reported in different literature (8752131,15965697).

实验方案

Product Specific Protocols
IF protocol for TAU antibody 82846-5-RRDownload protocol
IHC protocol for TAU antibody 82846-5-RRDownload protocol
WB protocol for TAU antibody 82846-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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