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验证数据展示

  • WB analysis using 82846-5-RR (same clone as 82846-5-PBS)

    WB analysis using 82846-5-RR (same clone as 82846-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82846-5-RR (TAU antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82846-5-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 82846-5-RR (same clone as 82846-5-PBS)

    IHC staining of human colon using 82846-5-RR (same clone as 82846-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82846-5-RR (TAU antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82846-5-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 82846-5-RR (same clone as 82846-5-PBS)

    IHC staining of human colon using 82846-5-RR (same clone as 82846-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82846-5-RR (TAU antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82846-5-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 82846-5-RR (same clone as 82846-5-PBS)

    IF Staining of mouse brain using 82846-5-RR (same clone as 82846-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TAU antibody (82846-5-RR, Clone: 252452C8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82846-5-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 82846-5-RR (same clone as 82846-5-PBS)

    IF Staining of mouse brain using 82846-5-RR (same clone as 82846-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TAU antibody (82846-5-RR, Clone: 252452C8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82846-5-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 82846-5-RR (same clone as 82846-5-PBS)

    IF Staining of mouse brain using 82846-5-RR (same clone as 82846-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using TAU antibody (82846-5-RR, Clone: 252452C8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82846-5-PBS in a different storage buffer formulation.

  • Affinity of 82846-5-RR

    Affinity of 82846-5-RR

    Biolayer interferometry (BLI) kinetic assays of 82846-5-RR against Human TAU were performed. The affinity constant is below 1 pM.

TAU Recombinant monoclonal antibody, PBS Only

TAU Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, IF-Fro, Indirect ELISA
Cat No. 82846-5-PBS

产品说明书

CloneNo. 252452C8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, IF-Fro, Indirect ELISA

别名

MAPT, AT180, DDPAC, MAPTL, Microtubule-associated protein tau

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

82846-5-PBS targets TAU in WB, IHC, IF-P, IF-Fro, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, IF-Fro, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Eg6232

Product name: Recombinant human TAU protein

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 1-758 aa of NM_016835.4

Sequence: MAEPRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDTDAGLKESPLQTPTEDGSEEPGSETSDAKSTPTAEDVTAPLVDEGAPGKQAAAQPHTEIPEGTTAEEAGIGDTPSLEDEAAGHVTQEPESGKVVQEGFLREPGPPGLSHQLMSGMPGAPLLPEGPREATRQPSGTGPEDTEGGRHAPELLKHQLLGDLHQEGPPLKGAGGKERPGSKEEVDEDRDVDESSPQDSPPSKASPAQDGRPPQTAAREATSIPGFPAEGAIPLPVDFLSKVSTEIPASEPDGPSVGRAKGQDAPLEFTFHVEITPNVQKEQAHSEEHLGRAAFPGAPGEGPEARGPSLGEDTKEADLPEPSEKQPAAAPRGKPVSRVPQLKARMVSKSKDGTGSDDKKAKTSTRSSAKTLKNRPCLSPKHPTPGSSDPLIQPSSPAVCPEPPSSPKYVSSVTSRTGSSGAKEMKLKGADGKTKIATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPKSGDRSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSSAKSRLQTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 microtubule-associated protein tau
别名 MAPT, AT180, DDPAC, MAPTL, Microtubule-associated protein tau
计算分子量79 kDa
观测分子量 45-70 kDa
GenBank蛋白编号NM_016835.4
基因名称 TAU
Gene ID (NCBI) 4137
RRIDAB_3743441
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP10636
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Tau (tubulin-associated unit) is a microtubule-associated protein (also known as MAPT), expressed mainly in neurons of the central nervous system. Its primary function is to modulate microtubule dynamics for maintaining axonal cytoskeleton. Various isoforms of Tau exist due to the alternative splicing, and short isoforms around 45-69 kDa and long isoforms around 100-110 kDa have been reported in different literature (8752131,15965697).

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