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CoraLite®594-conjugated TARS Monoclonal antibody

TARS Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1A11F10

Cat No : CL594-67828

Print datasheet

Synonyms

TARS, Threonine tRNA ligase, threonyl tRNA synthetase, ThrRS

验证数据展示

  • IF Staining of HeLa using CL594-67828

    IF Staining of HeLa using CL594-67828

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 TARS antibody (CL594-67828, Clone: 1A11F10 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67828-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67828 targets TARS in IF applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC
Tested Reactivity Human, mouse, rat
Immunogen TARS fusion protein Ag6614 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name threonyl-tRNA synthetase
Synonyms TARS, Threonine tRNA ligase, threonyl tRNA synthetase, ThrRS
Calculated Molecular Weight 83 kDa
Observed Molecular Weight 83-87 kDa
GenBank Accession NumberBC000517
Gene Symbol TARS
Gene ID (NCBI) 6897
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP26639
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

TARS (Threonyl-tRNA synthetase), also known as threonine-tRNA ligase or ThrRS, belongs to the aminoacyl-tRNA synthetase family. Aminoacyl-tRNA synthetase (aaRS) catalyzes the specific esterification of an amino acid with the 3'-terminal hydroxyl group of its cognate tRNA. Specificity of aminoacylation catalyzed by aaRSs ensures the accurate translation of the genetic code in the first step of protein synthesis (PMID: 26324710). TARS has some isoforms with the molecular mass of 83, 87 kDa and other MW.

实验方案

Product Specific Protocols
IF protocol for CL594 TARS antibody CL594-67828Download protocol
Standard Protocols
Click here to view our Standard Protocols