TAP1 Recombinant antibody

TAP1 Uni-rAbTM Recombinant Antibody for WB, IHC, IP, ELISA
Cat No. 85766-4-RR

产品说明书

CloneNo. 250037E7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, ELISA

ABCB2, ATP-binding cassette sub-family B member 2, EC:7.4.2.14, PSF1, PSF-1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inHeLa cells, HCT 116 cells, THP-1 cells, HepG2 cells, mouse spleen tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TAP1 Ready-To-Use IHC Kit. TAP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85766-4-RR targets TAP1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 TAP1 fusion protein Ag1619 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transporter 1, ATP-binding cassette, sub-family B (MDR/TAP)
别名 ABCB2, ATP-binding cassette sub-family B member 2, EC:7.4.2.14, PSF1, PSF-1
计算分子量 81 kDa
观测分子量 70-80 kDa
GenBank蛋白编号BC014081
基因名称 TAP1
Gene ID (NCBI) 6890
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ03518
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TAP1, also known as ABCB2, PSF1 or RING4, is a member of the ATP-binding cassette (ABC) family of transmembrane transporters and is an essential component of the major histocompatability complex (MHC) class I antigen-presenting pathway. TAP is involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. It also acts as a molecular scaffold for the final stage of MHC class I folding. Defects in TAP1 are a cause of bare lymphocyte syndrome type 1 (BLS1). Western blot analysis using this antibody detected a major band around 70-80 kDa in HeLa cells.

实验方案

Product Specific Protocols
WB protocol for TAP1 antibody 85766-4-RRDownload protocol
IHC protocol for TAP1 antibody 85766-4-RRDownload protocol
IP protocol for TAP1 antibody 85766-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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