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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 85766-4-RR (same clone as 85766-4-PBS)

    WB analysis using 85766-4-RR (same clone as 85766-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85766-4-RR (TAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85766-4-PBS in a different storage buffer formulation.

  • WB analysis of THP-1 using 85766-4-RR (same clone as 85766-4-PBS)

    WB analysis of THP-1 using 85766-4-RR (same clone as 85766-4-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 85766-4-RR (TAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85766-4-PBS in a different storage buffer formulation.

  • WB analysis of HCT 116 using 85766-4-RR (same clone as 85766-4-PBS)

    WB analysis of HCT 116 using 85766-4-RR (same clone as 85766-4-PBS)

    HCT 116 cells were subjected to SDS PAGE followed by western blot with 85766-4-RR (TAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85766-4-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 85766-4-RR (same clone as 85766-4-PBS)

    IP experiment of HepG2 using 85766-4-RR (same clone as 85766-4-PBS)

    IP result of anti-TAP1 (IP:85766-4-RR, 4ug; Detection:85766-4-RR 1:1000) with HepG2 cells lysate 1800 ug. This data was developed using the same antibody clone with 85766-4-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 85766-4-RR (same clone as 85766-4-PBS)

    IHC staining of human pancreas cancer using 85766-4-RR (same clone as 85766-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 85766-4-RR (TAP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85766-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TAP1 antibody included

TAP1 Recombinant antibody, PBS Only

TAP1 Uni-rAbTM Recombinant Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 85766-4-PBS

产品说明书

CloneNo. 250037E7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, Indirect ELISA

别名

ABCB2, ATP-binding cassette sub-family B member 2, EC:7.4.2.14, PSF1, PSF-1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy TAP1 Ready-To-Use IHC Kit. TAP1 primary antibody included.

产品信息

85766-4-PBS targets TAP1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 TAP1 fusion protein Ag1619 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transporter 1, ATP-binding cassette, sub-family B (MDR/TAP)
别名 ABCB2, ATP-binding cassette sub-family B member 2, EC:7.4.2.14, PSF1, PSF-1
计算分子量 81 kDa
观测分子量 70-80 kDa
GenBank蛋白编号BC014081
基因名称 TAP1
Gene ID (NCBI) 6890
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ03518
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TAP1, also known as ABCB2, PSF1 or RING4, is a member of the ATP-binding cassette (ABC) family of transmembrane transporters and is an essential component of the major histocompatability complex (MHC) class I antigen-presenting pathway. TAP is involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. It also acts as a molecular scaffold for the final stage of MHC class I folding. Defects in TAP1 are a cause of bare lymphocyte syndrome type 1 (BLS1). Western blot analysis using this antibody detected a major band around 70-80 kDa in HeLa cells.