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验证数据展示

  • WB analysis using 85766-4-RR (same clone as 85766-4-PBS)

    WB analysis using 85766-4-RR (same clone as 85766-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85766-4-RR (TAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85766-4-PBS in a different storage buffer formulation.

  • WB analysis of THP-1 using 85766-4-RR (same clone as 85766-4-PBS)

    WB analysis of THP-1 using 85766-4-RR (same clone as 85766-4-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 85766-4-RR (TAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85766-4-PBS in a different storage buffer formulation.

  • WB analysis of HCT 116 using 85766-4-RR (same clone as 85766-4-PBS)

    WB analysis of HCT 116 using 85766-4-RR (same clone as 85766-4-PBS)

    HCT 116 cells were subjected to SDS PAGE followed by western blot with 85766-4-RR (TAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85766-4-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 85766-4-RR (same clone as 85766-4-PBS)

    IP experiment of HepG2 using 85766-4-RR (same clone as 85766-4-PBS)

    IP result of anti-TAP1 (IP:85766-4-RR, 4ug; Detection:85766-4-RR 1:1000) with HepG2 cells lysate 1800 ug. This data was developed using the same antibody clone with 85766-4-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 85766-4-RR (same clone as 85766-4-PBS)

    IHC staining of human pancreas cancer using 85766-4-RR (same clone as 85766-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 85766-4-RR (TAP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85766-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TAP1 antibody included
  • Affinity and Kinetic Characterization of 85766-4-RR

    Affinity and Kinetic Characterization of 85766-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85766-4-RR against Human TAP1 were performed. The affinity constant is below 1 pM.

TAP1 Recombinant monoclonal antibody, PBS Only

TAP1 Uni-rAb® Recombinant Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 85766-4-PBS

产品说明书

CloneNo. 250037E7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, Indirect ELISA

别名

ABCB2, ATP-binding cassette sub-family B member 2, EC:7.4.2.14, PSF1, PSF-1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy TAP1 Ready-To-Use IHC Kit. TAP1 primary antibody included.

产品信息

85766-4-PBS targets TAP1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag1619

Product name: Recombinant human TAP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 509-808 aa of BC014081

Sequence: LYQMQFTQAVEVLLSIYPRVQKAVGSSEKIFEYLDRTPRCPPSGLLTPLHLEGLVQFQDVSFAYPNRPDVLVLQGLTFTLRPGEVTALVGPNGSGKSTVAALLQNLYQPTGGQLLLDGKPLPQYEHRYLHRQVAAVGQEPQVFGRSLQENIAYGLTQKPTMEEITAAAVKSGAHSFISGLPQGYDTEVDEAGSQLSGGQRQAVALARALIRKPCVLILDDATSALDANSQLQVEQLLYESPERYSRSVLLITQHLSLVEQADHILFLEGGAIREGGTHQQLMEKKGCYWAMVQAPADAPE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transporter 1, ATP-binding cassette, sub-family B (MDR/TAP)
别名 ABCB2, ATP-binding cassette sub-family B member 2, EC:7.4.2.14, PSF1, PSF-1
计算分子量 81 kDa
观测分子量 70-80 kDa
GenBank蛋白编号BC014081
基因名称 TAP1
Gene ID (NCBI) 6890
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ03518
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TAP1, also known as ABCB2, PSF1 or RING4, is a member of the ATP-binding cassette (ABC) family of transmembrane transporters and is an essential component of the major histocompatability complex (MHC) class I antigen-presenting pathway. TAP is involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. It also acts as a molecular scaffold for the final stage of MHC class I folding. Defects in TAP1 are a cause of bare lymphocyte syndrome type 1 (BLS1). Western blot analysis using this antibody detected a major band around 70-80 kDa in HeLa cells.

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