验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, THP-1 cells, Daudi cells, Raji cells, Ramos cells |
| Positive IP detected in | THP-1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85138-3-RR targets TANK in WB, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag25740 Product name: Recombinant human TANK protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-119 aa of BC003388 Sequence: MDKNIGEQLNKAYEAFRQACMDRDSAVKELQQKTENYEQRIREQQEQLSLQQTIIDKLKSQLLLVNSTQDNNYGCVPLLEDSETRKNNLTLDQPQDKVISGIAREKLPKVDIASAESSI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | TRAF family member-associated NFKB activator |
| 别名 | I TRAF, ITRAF, I-TRAF, TRAF family member-associated NF-kappa-B activator, TRAF interacting protein |
| 计算分子量 | 14 kDa |
| 观测分子量 | 48 kDa |
| GenBank蛋白编号 | BC003388 |
| 基因名称 | TANK |
| Gene ID (NCBI) | 10010 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q92844 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for TANK antibody 85138-3-RR | Download protocol |
| WB protocol for TANK antibody 85138-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
