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验证数据展示

  • WB analysis of Raji using 55317-1-AP

    WB analysis of Raji using 55317-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 55317-1-AP (TAL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of Raji using 55317-1-AP

    IP experiment of Raji using 55317-1-AP

    IP result of anti-TAL1 (IP:55317-1-AP, 4ug; Detection:55317-1-AP 1:1000) with Raji cells lysate 2000ug.

TAL1 Polyclonal antibody

TAL1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 55317-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IP, ELISA

别名

bHLHa17, SCL, Stem cell protein, tal 1, TAL1, TCL5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inRaji cells, Jurkat cells
Positive IP detected inRaji cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

55317-1-AP targets TAL1 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human, zebrafish
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 T-cell acute lymphocytic leukemia 1
别名 bHLHa17, SCL, Stem cell protein, tal 1, TAL1, TCL5
计算分子量 34 kDa
观测分子量 40-44 kDa
GenBank蛋白编号NM_003189
基因名称 TAL1
Gene ID (NCBI) 6886
RRIDAB_2881306
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP17542
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

T-cell acute lymphocytic leukemia protein 1 (TAL1) also named Stem cell protein,T-cell leukemia/lymphoma protein 5.It is specifically expressed in leukemic stem cell,implicated in the genesis of hemopoietic malignancies. It may play an important role in hemopoietic differentiation, serving as a positive regulator of erythroid differentiation. The 38kd, 44kd bands recognized by antibody are the phosphorylated forms of TAL1 and it's isoforms.

实验方案

Product Specific Protocols
WB protocol for TAL1 antibody 55317-1-APDownload protocol
IP protocol for TAL1 antibody 55317-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Pharmaceuticals (Basel)

Caulis Polygoni Multiflori Accelerates Megakaryopoiesis and Thrombopoiesis via Activating PI3K/Akt and MEK/ERK Signaling Pathways

Authors - Xin Yang
View Article
zebrafishWB

Development

BF170 hydrochloride enhances the emergence of hematopoietic stem and progenitor cells

Authors - WenYe Liu
View Article