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验证数据展示

  • WB analysis of Recombinant protein using 84959-4-RR

    WB analysis of Recombinant protein using 84959-4-RR

    Recombinant protein were subjected to SDS PAGE followed by western blot with 84959-4-RR (T7 RNA polymerase antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84959-4-RR

    Affinity and Kinetic Characterization of 84959-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84959-4-RR against T7 RNA polymerase were performed. The affinity constant is 1.84 nM.

    {{ptg:ProductFeaturesInfo}}

T7 RNA polymerase Recombinant monoclonal antibody

T7 RNA polymerase Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 84959-4-RR

产品说明书

CloneNo. 242544H3

宿主/亚型

Rabbit / IgG

种属反应性

bacteriophage t7

应用

WB, ELISA

别名

T7 polymerase, 242544H3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inRecombinant protein

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84959-4-RR targets T7 RNA polymerase in WB, ELISA applications and shows reactivity with bacteriophage t7 samples.

经测试应用 WB, ELISA Application Description
经测试反应性 bacteriophage t7
免疫原

CatNo: Ag32001

Product name: Recombinant bacteriophage t7 T7 RNA polymerase protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-300 aa of NP_041960

Sequence: MNTINIAKNDFSDIELAAIPFNTLADHYGERLAREQLALEHESYEMGEARFRKMFERQLKAGEVADNAAAKPLITTLLPKMIARINDWFEEVKAKRGKRPTAFQFLQEIKPEAVAYITIKTTLACLTSADNTTVQAVASAIGRAIEDEARFGRIRDLEAKHFKKNVEEQLNKRVGHVYKKAFMQVVEADMLSKGLLGGEAWSSWHKEDSIHVGVRCIEMLIESTGMVSLHRQNAGVVGQDSETIELAPEYAEAIATRAGALAGISPMFQPCVVPPKPWTGITGGGYWANGRRPLALVRTH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 T7 RNA polymerase
别名 T7 polymerase, 242544H3
计算分子量99 kDa
GenBank蛋白编号NP_041960
基因名称 T7 RNA polymerase
Gene ID (NCBI) 1261050
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP00573
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for T7 RNA polymerase antibody 84959-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols