验证数据展示
经过测试的应用
| Positive WB detected in | Recombinant protein |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
29943-1-AP targets T7 RNA polymerase in WB, ELISA applications and shows reactivity with bacteriophage t7 samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | bacteriophage t7 |
| 文献引用反应性 | hamster |
| 免疫原 |
CatNo: Ag32001 Product name: Recombinant bacteriophage t7 T7 RNA polymerase protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-300 aa of NP_041960 Sequence: MNTINIAKNDFSDIELAAIPFNTLADHYGERLAREQLALEHESYEMGEARFRKMFERQLKAGEVADNAAAKPLITTLLPKMIARINDWFEEVKAKRGKRPTAFQFLQEIKPEAVAYITIKTTLACLTSADNTTVQAVASAIGRAIEDEARFGRIRDLEAKHFKKNVEEQLNKRVGHVYKKAFMQVVEADMLSKGLLGGEAWSSWHKEDSIHVGVRCIEMLIESTGMVSLHRQNAGVVGQDSETIELAPEYAEAIATRAGALAGISPMFQPCVVPPKPWTGITGGGYWANGRRPLALVRTH 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | T7 RNA polymerase |
| 别名 | T7 polymerase |
| 计算分子量 | 99 kDa |
| GenBank蛋白编号 | NP_041960 |
| 基因名称 | T7 RNA polymerase |
| Gene ID (NCBI) | 1261050 |
| RRID | AB_2923624 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P00573 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for T7 RNA polymerase antibody 29943-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Adv Discovery of diverse and high-quality mRNA capping enzymes through a language model-enabled platform | ||
Virol Sin Efficient and robust reverse genetics system for bovine rotavirus generation and its application for antiviral screening |