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验证数据展示

  • IF Staining of mouse brain using CL594-66437

    IF Staining of mouse brain using CL594-66437

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 Syntaxin 1B antibody (CL594-66437, Clone: 2D3B5 ) at dilution of 1:200.

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CoraLite®594-conjugated Syntaxin 1A / Syntaxin 1B Monoclonal antibody

Syntaxin 1A / Syntaxin 1B Monoclonal Antibody for IF-P
Cat No. CL594-66437

产品说明书

CloneNo. 2D3B5

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF-P

别名

Syntaxin 1B, STX1B, Syntaxin 1A, 2D3B5, STX1B1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive IF-P detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy Syntaxin 1B Ready-To-Use IHC Kit. Syntaxin 1B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66437-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66437 targets Syntaxin 1A / Syntaxin 1B in IF-P applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse, rat
免疫原 Syntaxin 1A / Syntaxin 1B fusion protein Ag10257 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 syntaxin 1B
别名 Syntaxin 1B, STX1B, Syntaxin 1A, 2D3B5, STX1B1
计算分子量 288 aa, 33 kDa
观测分子量 33 kDa
GenBank蛋白编号BC062298
基因名称 STX1B
Gene ID (NCBI) 112755
RRIDAB_2919991
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP61266
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Syntaxins are a family of transmembrane proteins that belong to the SNARE complex (PMID: 11737951). In conjunction with other SNAREs and with the cytoplasmic NSF and SNAP proteins, syntaxins mediate vesicle fusion in diverse vesicular transport processes along the exocytic and the endocytic pathway (PMID: 11737951). Syntaxin 1A and 1B, two closely related isoforms of syntaxin 1, are involved in synaptic vesicle docking and fusion during neurotransmitter release (PMID: 7690687; 1321498; 18691641).

实验方案

Product Specific Protocols
IF protocol for CL594 Syntaxin 1A / Syntaxin 1B antibody CL594-66437Download protocol
Standard Protocols
Click here to view our Standard Protocols