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验证数据展示

  • WB analysis using 68220-1-Ig

    WB analysis using 68220-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68220-1-Ig (Syntaxin 18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

Syntaxin 18 Monoclonal antibody

Syntaxin 18 Monoclonal Antibody for WB, ELISA
Cat No. 68220-1-Ig

产品说明书

CloneNo. 2A2C9

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse, rat, pig

应用

WB, ELISA

别名

DKFZp686O15149, STX18, syntaxin 18, Ufe1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inLNCaP cells, rat brain tissue, pig brain tissue, HEK-293 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, mouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68220-1-Ig targets Syntaxin 18 in WB, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, mouse, rat, pig
免疫原

CatNo: Ag8947

Product name: Recombinant human Syntaxin 18 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-317 aa of BC014613

Sequence: MAVDITLLFRASVKTVKTRNKALGVAVGGGVDGSRDELFRRSPRPKGDFSSRAREVISHIGKLRDFLLEHRKDYINAYSHTMSEYGRMTDTERDQIDQDAQIFMRTCSEAIQQLRTEAHKEIHSQQVKEHRTAVLDFIEDYLKRVCKLYSEQRAIRVKRVVDKKRLSKLEPEPNTKTRESTSSEKVSQSPSKDSEENPATEERPEKILAETQPELGTWGDGKGEDELSPEEIQMFEQENQRLIGEMNSLFDEVRQIEGRVVEISRLQEIFTEKVLQQEAEIDSIHQLVVGATENIKEGNEDIREAIKNNAGFRVWIL

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 syntaxin 18
别名 DKFZp686O15149, STX18, syntaxin 18, Ufe1
计算分子量 335 aa, 39 kDa
观测分子量39 kDa
GenBank蛋白编号BC014613
基因名称 Syntaxin 18
Gene ID (NCBI) 53407
RRIDAB_2935308
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9P2W9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Syntaxin 18 is a novel member of the syntaxin family and it is involved in ER-mediated phagocytosis by regulating the specific and direct fusion of the ER and plasma or phagosomal membranes( PMID: 16790498).

实验方案

Product Specific Protocols
WB protocol for Syntaxin 18 antibody 68220-1-IgDownload protocol
FC protocol for Syntaxin 18 antibody 68220-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols