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验证数据展示

  • WB analysis using 84452-1-RR (same clone as 84452-1-PBS)

    WB analysis using 84452-1-RR (same clone as 84452-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84452-1-RR (Synaptotagmin-5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84452-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 84452-1-RR (same clone as 84452-1-PBS)

    IHC staining of mouse brain using 84452-1-RR (same clone as 84452-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84452-1-RR (Synaptotagmin-5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84452-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 84452-1-RR (same clone as 84452-1-PBS)

    IHC staining of mouse brain using 84452-1-RR (same clone as 84452-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84452-1-RR (Synaptotagmin-5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84452-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 84452-1-RR (same clone as 84452-1-PBS)

    IF Staining of mouse brain using 84452-1-RR (same clone as 84452-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Synaptotagmin-5 antibody (84452-1-RR, Clone: 241452E3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84452-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84452-1-RR

    Affinity and Kinetic Characterization of 84452-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84452-1-RR against Human Synaptotagmin-5 were performed. The affinity constant is 0.131 nM.

Synaptotagmin-5 Recombinant monoclonal antibody, PBS Only

Synaptotagmin-5 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 84452-1-PBS

产品说明书

CloneNo. 241452E3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

SytV, SYT5, Synaptotagmin V, 241452E3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84452-1-PBS targets Synaptotagmin-5 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12677

Product name: Recombinant human SYT5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 51-386 aa of BC046157

Sequence: YRKSCRRRTGKKSQAQAQVHLQEVKGLGQSYIDKVQPEVEELEPAPSGPGQQVADKHELGQLQYSLDYDFQSGQLLVGILQAMGLAALDLGGSSDPYVRVYLLPDKRRRYETKVHRQTLNPHFGETFAFKVPYVELGGRVLVMAVYDFDRFSRNDAIGEVRVPMSSVDLGRPVQAWRELQAAPREEQEKLGDICFSLRYVPTAGKLTVIVLEAKNLKKMDVGGLSDPYVKVHLLQGGKKVRKKKTTIKKNTLNPYYNEAFSFEVPCDQVQKVQVELTVLDYDKLGKNEAIGRVAVGAAAGGAGLRHWADMLANPRRPIAQWHSLRPPDRVRLLPAP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 synaptotagmin V
别名 SytV, SYT5, Synaptotagmin V, 241452E3
计算分子量 43 kDa
观测分子量42 kDa
GenBank蛋白编号BC046157
基因名称 Synaptotagmin-5
Gene ID (NCBI) 6861
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDO00445
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). Synaptotagmin-5 (SYT5) belongs to the synaptotagmin family. Synaptotagmin-5 can function as an exocytosis regulator (PMID: 27793683) and is a protein potentially associated with the pathology of schizophrenia (PMID: 32860155).

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