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验证数据展示

  • WB analysis using 28667-1-AP (same clone as 28667-1-PBS)

    WB analysis using 28667-1-AP (same clone as 28667-1-PBS)

    mouse brain tissue and rat brain tissue were subjected to SDS PAGE followed by western blot with 28667-1-AP (Synaptotagmin-3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28667-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 28667-1-AP (same clone as 28667-1-PBS)

    IHC staining of mouse brain using 28667-1-AP (same clone as 28667-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28667-1-AP (Synaptotagmin-3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28667-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 28667-1-AP (same clone as 28667-1-PBS)

    IHC staining of mouse brain using 28667-1-AP (same clone as 28667-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28667-1-AP (Synaptotagmin-3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28667-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 28667-1-AP (same clone as 28667-1-PBS)

    IF Staining of mouse brain using 28667-1-AP (same clone as 28667-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Synaptotagmin-3 antibody (28667-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28667-1-PBS in a different storage buffer formulation.

Synaptotagmin-3 Polyclonal antibody, PBS Only

Synaptotagmin-3 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 28667-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

28667-1-PBS targets Synaptotagmin-3 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag30027

Product name: Recombinant human SYT3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 85-330 aa of BC028379

Sequence: DKGGSAVGGGPLRKDLGPGVGLAGLVGGGGHHLAAGLGGHPLLGGPHHHAHAAHHPPFAELLEPGSLGGSDTPEPSYLDMDSYPEAAAAAVAAGVKPSQTSPELPSEGGAGSGLLLLPPSGGGLPSAQSHQQVTSLAPTTRYPALPRPLTQQTLTSQPDPSSEERPPALPLPLPGGEEKAKLIGQIKPELYQGTGPGGRRSGGGPGSGEAGTGAPCGRISFALRYLYGSDQLVVRILQAMDLPAKD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 synaptotagmin III
别名
计算分子量 590 aa, 63 kDa
观测分子量70-75 kDa
GenBank蛋白编号BC028379
基因名称 Synaptotagmin-3
Gene ID (NCBI) 84258
RRIDAB_2918187
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BQG1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). Synaptotagmin-3 (SYT3) is highly expressed in brain, adipose tissue, and heart (PMID: 30545844). Synaptotagmin-3 is highly enriched in synaptic plasma membranes (PMID: 10373432). It has been reported that synaptotagmin-3 internalizes AMPA receptors to depress synaptic strength and promote forgetting (PMID: 30545844).

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