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CoraLite® Plus 488-conjugated Synaptopodin Polyclonal antibody

Synaptopodin Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra), IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-21064

Print datasheet

Synonyms

KIAA1029, synaptopodin, SYNPO

验证数据展示

  • IF Staining of rat brain using CL488-21064

    IF Staining of rat brain using CL488-21064

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite® Plus 488 Synaptopodin antibody (CL488-21064) at dilution of 1:200.

  • FC experiment of U-87 MG using CL488-21064

    FC experiment of U-87 MG using CL488-21064

    1X10^6 U-87 MG cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Synaptopodin (CL488-21064) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inrat brain tissue
Positive FC detected inU-87 MG cells
For other applications, we recommend the unconjugated version of this antibody, 21064-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-21064 targets Synaptopodin in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra), IF
Tested Reactivity human, mouse, rat
Immunogen Synaptopodin fusion protein Ag15089 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name synaptopodin
Synonyms KIAA1029, synaptopodin, SYNPO
Calculated Molecular Weight 929 aa, 99 kDa
Observed Molecular Weight 99-115 kDa
GenBank Accession NumberBC146665
Gene Symbol SYNPO
Gene ID (NCBI) 11346
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N3V7
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Synaptopodin is a actin-associated protein expressed in mature dendritic spines and renal podocytes. Several isoforms of synaptopodin have been reported: the 100 kDa neural form and 110 kDa renal form. 44-45 kDa may represent the degradation product of synaptopodin. As a podocyte specific protein, synaptopodin expression level was decreased in kidney but increased in the urinary podocytes from patients with preeclampsia, indicating that synaptopodin is a useful marker for preeclampsia. (15841212, 10555072)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Synaptopodin antibody CL488-21064Download protocol
Standard Protocols
Click here to view our Standard Protocols