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验证数据展示

  • FC experiment of human PBMCs using 98624-2-RR (same clone as 98624-2-PBS)

    FC experiment of human PBMCs using 98624-2-RR (same clone as 98624-2-PBS)

    1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human Survivin Rabbit RecAb (98624-2-RR, Clone: 251227E5) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD14 (26IC) (CL647-65246, Clone: 26IC). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). Cells were incubated with FC Receptor Block prior to staining. This data was developed using the same antibody clone with 98624-2-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 98624-2-RR (same clone as 98624-2-PBS)

    FC experiment of human PBMCs using 98624-2-RR (same clone as 98624-2-PBS)

    1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human Survivin Rabbit RecAb (98624-2-RR, Clone: 251227E5) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). Cells were incubated with FC Receptor Block prior to staining. This data was developed using the same antibody clone with 98624-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98624-2-RR

    Affinity and Kinetic Characterization of 98624-2-RR

    Biolayer interferometry (BLl) kinetic assays of 98624-2-RR against Human Survivin were performed. The affinity constant is 0.887 nM.

Anti-Human Survivin Rabbit Recombinant Antibody, PBS Only

Survivin Uni-rAb® Recombinant Antibody for FC (Intra)
Cat No. 98624-2-PBS

产品说明书

CloneNo. 251227E5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra)

别名

BIRC5, Baculoviral IAP repeat-containing protein 5, BIRC 5, EPR 1, IAP4

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SURVIVIN Ready-To-Use IHC Kit. SURVIVIN primary antibody included.

产品信息

98624-2-PBS targets Survivin in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原

CatNo: Eg4645

Product name: Recombinant human Survivin protein

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 1-142 aa of /

Sequence: MGAPTLPPAWQPFLKDHRISTFKNWPFLEGCACTPERMAEAGFIHCPTENEPDLAQCFFCFKELEGWEPDDDPIEEHKKHSSGCAFLSVKKQFEELTLGEFLKLDRERAKNKIAKETNNKKKEFEETAKKVRRAIEQLAAMD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 baculoviral IAP repeat-containing 5
别名 BIRC5, Baculoviral IAP repeat-containing protein 5, BIRC 5, EPR 1, IAP4
计算分子量16kDa
GenBank蛋白编号/
基因名称 Survivin
Gene ID (NCBI) 332
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO15392-1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Survivin, also called BIRC5, is a unique member of the inhibitor of apoptosis (IAP) protein family. Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy, but is completely absent in terminally differentiated cells. The differential expression of survivin in cancer versus normal tissues makes it a useful tool in cancer diagnosis and a promising therapeutic target. Survivin expression is also highly regulated by the cell cycle and is only expressed in the G2-M phase. It is known that survivin localizes to the mitotic spindle by interaction with tubulin during mitosis and may play a contributing role in regulating mitosis. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. It also is a direct target gene of the Wnt pathway and is upregulated by beta-catenin.

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