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验证数据展示

  • IF Staining of MCF-7 using 98624-1-RR

    IF Staining of MCF-7 using 98624-1-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Survivin antibody (98624-1-RR, Clone: 251227G1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of human PBMCs using 98624-1-RR

    FC experiment of human PBMCs using 98624-1-RR

    1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human Survivin Rabbit RecAb (98624-1-RR, Clone:251227G1),or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9)and PE-Conjugated Goat Anti-Rabbit IgG(H+L),Cells were then stained with CoraLite® Plus 647 Anti-Human CD14 (26IC) (CL647-65246, Clone: 26IC),Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).Cells were incubated with FC Receptor Block prior to staining.

  • IF Staining of A549 using 98624-1-RR

    IF Staining of A549 using 98624-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using Survivin antibody (98624-1-RR, Clone: 251227G1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of human PBMCs using 98624-1-RR

    FC experiment of human PBMCs using 98624-1-RR

    1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human Survivin Rabbit RecAb (98624-1-RR, Clone:251227G1)(red),or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9)(blue)and PE-Conjugated Goat Anti-Rabbit IgG(H+L).Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).Cells were incubated with FC Receptor Block prior to staining.

  • Affinity of 98624-1-RR

    Affinity of 98624-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98624-1-RR against Human Survivin were performed. The affinity constant is 3.16 nM.

Anti-Human Survivin Rabbit Recombinant Antibody

Survivin Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. 98624-1-RR

产品说明书

CloneNo. 251227G1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

BIRC5, Baculoviral IAP repeat-containing protein 5, BIRC 5, EPR 1, IAP4

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells, A549 cells
Positive FC (Intra) detected inFC receptor blocked human PBMCs
Planning an IHC experiment? We recommend our IHCeasy SURVIVIN Ready-To-Use IHC Kit. SURVIVIN primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98624-1-RR targets Survivin in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原

CatNo: Eg4645

Product name: Recombinant human Survivin protein

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 1-142 aa of /

Sequence: MGAPTLPPAWQPFLKDHRISTFKNWPFLEGCACTPERMAEAGFIHCPTENEPDLAQCFFCFKELEGWEPDDDPIEEHKKHSSGCAFLSVKKQFEELTLGEFLKLDRERAKNKIAKETNNKKKEFEETAKKVRRAIEQLAAMD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 baculoviral IAP repeat-containing 5
别名 BIRC5, Baculoviral IAP repeat-containing protein 5, BIRC 5, EPR 1, IAP4
计算分子量16kDa
GenBank蛋白编号/
基因名称 Survivin
Gene ID (NCBI) 332
RRIDAB_3746223
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO15392-1
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

Survivin, also called BIRC5, is a unique member of the inhibitor of apoptosis (IAP) protein family. Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy, but is completely absent in terminally differentiated cells. The differential expression of survivin in cancer versus normal tissues makes it a useful tool in cancer diagnosis and a promising therapeutic target. Survivin expression is also highly regulated by the cell cycle and is only expressed in the G2-M phase. It is known that survivin localizes to the mitotic spindle by interaction with tubulin during mitosis and may play a contributing role in regulating mitosis. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. It also is a direct target gene of the Wnt pathway and is upregulated by beta-catenin.

实验方案

Product Specific Protocols
FC protocol for Survivin antibody 98624-1-RRDownload protocol
IF protocol for Survivin antibody 98624-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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