ChromoTek Spot-Trap® Magnetic Particles M-270 Kit

Spot-Trap® Magnetic Particles M-270 are affinity beads for immunoprecipitation (IP) of Spot-tagged proteins It comprises an anti-Spot-Tag® VHH/ Nanobody coupled to Magnetic Particles M-270.

Specificity

Spot-Tag®

Applications

IP, CoIP, ChIP, RIP

Conjugate

Magnetic Particles M-270

Type

Nanobody

Cat No : etdk

Synonyms

etdk, etdk-20, Spot Trap Magnetic Particles M-270, Spot-Trap Magnetic Particles M-270, Spot-Trap Magnetic Particles M-270 Kit, Spot-Trap® Magnetic Particles M-270 Kit, etdk-20, PDRVRAVSHWSS, spot, spot tag, spot-tag, Spot-Trap® Magnetic Particles M-270



Product Information

Spot-Trap® Magnetic Particles M-270 are affinity beads for immunoprecipitation (IP) of Spot-tagged proteins It comprises an anti-Spot-Tag® VHH/ Nanobody coupled to Magnetic Particles M-270.

DescriptionThe Spot-Trap Magnetic Particles M-270 Kit contains Spot-Trap Magnetic Particles M-270, lysis, wash, and elution buffers for efficient immunoprecipitation of Spot-tagged proteins and their interacting factors.

• No contaminating heavy and light antibody chains

• Extraordinary low background compared to other affinity resins

• Recombinantly expressed without batch-to-batch variation

• Well-characterized and validated

• Compatible with harsh buffer conditions

• Suitable for downstream mass spec analysis

• Short incubation time of 30-60 minutes

ApplicationsIP, CoIP, ChIP, RIP
Specificity/TargetSpot-Tag ® sequence PDRVRAVSHWSS
Binding capacity2.25 μg of recombinant Spot-tagged protein (~30 kDa) per 25 μL bead slurry
ConjugateMagnetic Particles M-270, size: 2.8 µm
high throughput-compatible
Elution bufferSDS sample buffer
10 mM NaOH pH 12
Wash buffer compatibility2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 2 % Triton X-100
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 6 nM
Compatibility with mass spectrometryThe Spot-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2861252
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!
Spot-Tag® OriginEngineered 12 amino acid sequence modified from the unstructured N-terminus of beta-catenin
Cross ReactivityThe Spot-Nanobody shows minimal cross reactivity with beta-catenin.

Kit components

ComponentDescription
Lysis bufferOptimized for cytoplasmatic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer Removal of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate
Spot-Trap Magnetic Particles M-27020 reactions (500 µL)