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验证数据展示

  • FC experiment of human peripheral blood leukocytes using 98264-6-RR

    FC experiment of human peripheral blood leukocytes using 98264-6-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.125 ug Anti-Human Siglec-9 Rabbit RecAb (98264-6-RR, Clone: 241799C10) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human peripheral blood leukocytes using 98264-6-RR

    FC experiment of human peripheral blood leukocytes using 98264-6-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.125 ug Anti-Human Siglec-9 Rabbit RecAb (98264-6-RR, Clone: 241799C10) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98264-6-RR

    Affinity and Kinetic Characterization of 98264-6-RR

    Biolayer interferometry (BLl) kinetic assays of 98264-6-RR against Human Siglec-9 were performed. The affinity constant is 0.682 nM.

Anti-Human Siglec-9 Rabbit Recombinant Antibody

Siglec-9 Uni-rAbTM Recombinant Antibody for FC
Cat No. 98264-6-RR

产品说明书

CloneNo. 241799C10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

SIGLEC9, Siglec 9, Sialic acid-binding Ig-like lectin 9, Protein FOAP 9, CD329

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive FC detected inhuman peripheral blood leukocytes

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.13 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98264-6-RR targets Siglec-9 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sialic acid binding Ig-like lectin 9
别名 SIGLEC9, Siglec 9, Sialic acid-binding Ig-like lectin 9, Protein FOAP 9, CD329
计算分子量50 kDa
GenBank蛋白编号NM_014441.3
基因名称 Siglec-9
Gene ID (NCBI) 27180
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y336-1
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

Sialic acid binding Ig-like lectin 9 (Siglec-9), also known as CD329, is a member of the Siglec family of glycan-recognition proteins. Siglec-9 is a type-I transmembrane protein consisting of an N-terminal extracellular region that contains an N-terminal V-set domain and two C2-set domains, a transmembrane region, and an intracellular domain with an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an ITIM-like motif (PMID: 10801862). It is expressed quite broadly among human blood leukocytes, including monocytes, neutrophils, B cells, NK cells, and minor subsets of T cells (PMID: 32322597). Siglec-9 functions as an inhibitory immune checkpoint and can be targeted to enhance therapeutic antitumor immunity (PMID: 34155121; 37460871).

实验方案

Product Specific Protocols
FC protocol for Siglec-9 antibody 98264-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols