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验证数据展示

  • FC experiment of human peripheral blood leukocytes using 98264-6-RR

    FC experiment of human peripheral blood leukocytes using 98264-6-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human Siglec-9 Rabbit RecAb (98264-6-RR, Clone: 241799C10), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human peripheral blood leukocytes using 98264-6-RR

    FC experiment of human peripheral blood leukocytes using 98264-6-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human Siglec-9 Rabbit RecAb (98264-6-RR, Clone: 241799C10) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98264-6-RR

    Affinity and Kinetic Characterization of 98264-6-RR

    Biolayer interferometry (BLl) kinetic assays of 98264-6-RR against Human Siglec-9 were performed. The affinity constant is 0.682 nM.

Anti-Human Siglec-9 Rabbit Recombinant Antibody

Siglec-9 Uni-rAb® Recombinant Antibody for FC
Cat No. 98264-6-RR

产品说明书

CloneNo. 241799C10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

SIGLEC9, 241799C10, CD329, Protein FOAP 9, Sialic acid-binding Ig-like lectin 9

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive FC detected inhuman peripheral blood leukocytes

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98264-6-RR targets Siglec-9 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原

CatNo: Eg1084

Product name: Recombinant Human Siglec-9 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 18-348 aa of NM_014441.3

Sequence: QTSKLLTMQSSVTVQEGLCVHVPCSFSYPSHGWIYPGPVVHGYWFREGANTDQDAPVATNNPARAVWEETRDRFHLLGDPHTKNCTLSIRDARRSDAGRYFFRMEKGSIKWNYKHHRLSVNVTALTHRPNILIPGTLESGCPQNLTCSVPWACEQGTPPMISWIGTSVSPLDPSTTRSSVLTLIPQPQDHGTSLTCQVTFPGASVTTNKTVHLNVSYPPQNLTMTVFQGDGTVSTVLGNGSSLSLPEGQSLRLVCAVDAVDSNPPARLSLSWRGLTLCPSQPSNPGVLELPWVHLRDAAEFTCRAQNPLGSQQVYLNVSLQSKATSGVTQG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sialic acid binding Ig-like lectin 9
别名 SIGLEC9, 241799C10, CD329, Protein FOAP 9, Sialic acid-binding Ig-like lectin 9
计算分子量50 kDa
GenBank蛋白编号NM_014441.3
基因名称 Siglec-9
Gene ID (NCBI) 27180
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y336-1
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

Sialic acid binding Ig-like lectin 9 (Siglec-9), also known as CD329, is a member of the Siglec family of glycan-recognition proteins. Siglec-9 is a type-I transmembrane protein consisting of an N-terminal extracellular region that contains an N-terminal V-set domain and two C2-set domains, a transmembrane region, and an intracellular domain with an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an ITIM-like motif (PMID: 10801862). It is expressed quite broadly among human blood leukocytes, including monocytes, neutrophils, B cells, NK cells, and minor subsets of T cells (PMID: 32322597). Siglec-9 functions as an inhibitory immune checkpoint and can be targeted to enhance therapeutic antitumor immunity (PMID: 34155121; 37460871).

实验方案

Product Specific Protocols
FC protocol for Siglec-9 antibody 98264-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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