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验证数据展示

  • FC experiment of human PBMCs using 98416-1-RR

    FC experiment of human PBMCs using 98416-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Siglec-6/CD327 Rabbit RecAb (98416-1-RR, Clone: 243088G2) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 98416-1-RR

    FC experiment of human PBMCs using 98416-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Siglec-6/CD327 Rabbit RecAb (98416-1-RR, Clone: 243088G2) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated.

Anti-Human Siglec-6/CD327 Rabbit Recombinant Antibody

Siglec-6/CD327 Uni-rAbTM Recombinant Antibody for FC
Cat No. 98416-1-RR

产品说明书

CloneNo. 243088G2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

SIGLEC6, CD327, CD33 antigen like 1, CD33 antigen-like 1, CD33L

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98416-1-RR targets Siglec-6/CD327 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Siglec-6/CD327 fusion protein Eg2070 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sialic acid binding Ig-like lectin 6
别名 SIGLEC6, CD327, CD33 antigen like 1, CD33 antigen-like 1, CD33L
计算分子量 426 aa, 47 kDa
GenBank蛋白编号BC035359
基因名称 SIGLEC6
Gene ID (NCBI) 946
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO43699
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

Siglec-6, also known as CD327, originally identified on trophoblast cells of the placenta, is highly and consistently expressed on MCs from both mucosal and non-mucosal sites. Siglec-6 consists of 3 extracellular immunoglobulin (Ig) domains and 2 intracellular immunoreceptor tyrosine-based inhibition motifs (ITIMs), closely resembling the molecular structure of Siglec-3 (CD33) (PMID: 34289026). Siglec-6 was recruited to placental expression during human evolution, presumably to interact with sialylated ligands for specific negative signaling functions and/or to regulate leptin availability (PMID: 17580316). Siglec-6 is an immunoreceptor tyrosine-based inhibitory motif (ITIM)-bearing receptor selectively expressed by mast cells, making it a promising target for therapeutic intervention (PMID: 35406705).

实验方案

Product Specific Protocols
FC protocol for Siglec-6/CD327 antibody 98416-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols