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验证数据展示

  • IF Staining of HeLa using CL488-66188

    IF Staining of HeLa using CL488-66188

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 Septin 8 antibody (CL488-66188, Clone: 4A12G9 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated Septin 8 Monoclonal antibody

Septin 8 Monoclonal Antibody for IF/ICC
Cat No. CL488-66188

产品说明书

CloneNo. 4A12G9

宿主/亚型

Mouse / IgM

种属反应性

human

应用

IF/ICC

别名

KIAA0202, SEP2, SEPT8, septin 8

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

For other applications, we recommend the unconjugated version of this antibody, 66188-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66188 targets Septin 8 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原

CatNo: Ag19114

Product name: Recombinant human SEPT8 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-256 aa of BC001329

Sequence: MKKLDSKVNIIPIIAKADTISKSELHKFKIKIMGELVSNGVQIYQFPTDDEAVAEINAVMNAHLPFAVVGSTEEVKVGNKLVRARQYPWGVVQVENENHCDFVKLREMLIRVNMEDLREQTHSRHYELYRRCKLEEMGFQDSDGDSQPFSLQETYEAKRKEFLSELQRKEEEMRQMFVNKVKETELELKEKERELHEKFEHLKRVHQEEKRKVEEKRRELEEETNAFNRRKAAVEALQSQALHATSQQPLRKDKDK

种属同源性预测
宿主/亚型 Mouse / IgM
抗体类别 Monoclonal
产品类型 Antibody
全称 septin 8
别名 KIAA0202, SEP2, SEPT8, septin 8
计算分子量55.6 kDa
观测分子量 50-58 kDa
GenBank蛋白编号BC001329
基因名称 Septin 8
Gene ID (NCBI) 23176
RRIDAB_3084219
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Thiophilic affinity chromatograph
UNIPROT IDQ92599
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Septin 8, also named as KIAA0202, is a member of the highly conserved septin family. Septins are membrane-associated GTPases which are involved in cytokinesis and cellular morphogenesis. Septin 8 is an interaction partner of Septin 4. It may play a role in platelet secretion.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Septin 8 antibody CL488-66188Download protocol
Standard Protocols
Click here to view our Standard Protocols
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