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验证数据展示

  • WB analysis of human peripheral blood leukocyte using 83947-5-RR

    WB analysis of human peripheral blood leukocyte using 83947-5-RR

    human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 83947-5-RR (P-Selectin/CD62P antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 83947-5-RR

    IHC staining of human tonsillitis using 83947-5-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83947-5-RR (P-Selectin/CD62P antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 83947-5-RR

    IHC staining of human tonsillitis using 83947-5-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83947-5-RR (P-Selectin/CD62P antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 83947-5-RR

    IHC staining of human tonsillitis using 83947-5-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83947-5-RR (P-Selectin/CD62P antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human tonsillitis using 83947-5-RR

    IF Staining of human tonsillitis using 83947-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using P-Selectin/CD62P antibody (83947-5-RR, Clone: 240766D3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human tonsillitis using 83947-5-RR

    IF Staining of human tonsillitis using 83947-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using P-Selectin/CD62P antibody (83947-5-RR, Clone: 240766D3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83947-5-RR

    Affinity and Kinetic Characterization of 83947-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83947-5-RR against Mouse P-Selectin/CD62P were performed. The affinity constant is 7.97 nM.

P-Selectin/CD62P Recombinant antibody

P-Selectin/CD62P Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 83947-5-RR

产品说明书

CloneNo. 240766D3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

P-selectin, CD62P, 240766D3, PADGEM, P selectin

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman peripheral blood leukocyte
Positive IHC detected inhuman tonsillitis tissue, rat spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83947-5-RR targets P-Selectin/CD62P in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 selectin, platelet
别名 P-selectin, CD62P, 240766D3, PADGEM, P selectin
计算分子量83 kDa
观测分子量140 kDa
GenBank蛋白编号NM_011347.2
基因名称 Selp
Gene ID (NCBI) 20344
RRIDAB_3671527
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ01102
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

P-selectin, also known as GMP140 or CD62P, is a transmembrane glycoprotein that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is an adhesion molecule involved in the pathogenesis of inflammation, thrombosis, and oncogenesis. P-selectin is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.

实验方案

Product Specific Protocols
WB protocol for P-Selectin/CD62P antibody 83947-5-RRDownload protocol
IHC protocol for P-Selectin/CD62P antibody 83947-5-RRDownload protocol
IF protocol for P-Selectin/CD62P antibody 83947-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols