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验证数据展示

  • WB analysis of human peripheral blood leukocyte using 83947-5-RR (same clone as 83947-5-PBS)

    WB analysis of human peripheral blood leukocyte using 83947-5-RR (same clone as 83947-5-PBS)

    human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 83947-5-RR (P-Selectin/CD62P antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83947-5-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 83947-5-RR (same clone as 83947-5-PBS)

    IHC staining of human tonsillitis using 83947-5-RR (same clone as 83947-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83947-5-RR (P-Selectin/CD62P antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83947-5-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 83947-5-RR (same clone as 83947-5-PBS)

    IHC staining of human tonsillitis using 83947-5-RR (same clone as 83947-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83947-5-RR (P-Selectin/CD62P antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83947-5-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 83947-5-RR (same clone as 83947-5-PBS)

    IF Staining of human tonsillitis using 83947-5-RR (same clone as 83947-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using P-Selectin/CD62P antibody (83947-5-RR, Clone: 240766D3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83947-5-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 83947-5-RR (same clone as 83947-5-PBS)

    IF Staining of human tonsillitis using 83947-5-RR (same clone as 83947-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using P-Selectin/CD62P antibody (83947-5-RR, Clone: 240766D3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83947-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83947-5-RR

    Affinity and Kinetic Characterization of 83947-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83947-5-RR against Mouse P-Selectin/CD62P were performed. The affinity constant is 7.97 nM.

Mouse P-Selectin/CD62P Recombinant monoclonal antibody, PBS Only

P-Selectin/CD62P Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 83947-5-PBS

产品说明书

CloneNo. 240766D3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

CD62P, P-selectin, 240766D3, Grmp, P selectin

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83947-5-PBS targets P-Selectin/CD62P in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Eg0691

Product name: Recombinant Mouse Selp/P-selectin protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 42-709 aa of NM_011347.2

Sequence: WTYNYSTKAYSWNNSRVFCRRHFTDLVAIQNKNEIAHLNDVIPFFNSYYWIGIRKINNKWTWVGTNKTLTEEAENWADNEPNNKKNNQDCVEIYIKSNSAPGKWNDEPCFKRKRALCYTASCQDMSCSNQGECIETIGSYTCSCYPGFYGPECEYVKECGKVNIPQHVLMNCSHPLGEFSFNSQCTFSCAEGYELDGPGELQCLASGIWTNNPPKCDAVQCQSLEAPPHGTMACMHPIAAFAYDSSCKFECQPGYRARGSNTLHCTGSGQWSEPLPTCEAIACEPPEIPIHGSMDCVPSTGTFGYNSSCTFLCAEGFVLKGNDAIQCADSGQWTAPAPFCEALQCPEFPVPSKAQVNCSDPFGTLTYQSVCSFSCDEGSLLVGASVIRCLATGHWNGAPPECQAVSCAPMLSPENGSMTCVQPLGNSTYKSTCQFMCDEGFYLSGPERLDCSPSGHWTGTPPTCEAIKCPGIFAPEQGNLDCSHVHGEFGVGSICHFSCNEDFELLGSENVECTVSGRWSAPPPTCKGITSLPAPAVRCPALTTPGQGTMSCQHHLGSFGPNTTCYFGCKTGFTLRGANSLRCRASGQWTAVTPMCRAVKCSELHMDTAVAMNCSNPWGNFSYGSTCTFQCPEGQSLNGSVRATCREDGHWSDAMPTCQAGTLTIQEA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 selectin, platelet
别名 CD62P, P-selectin, 240766D3, Grmp, P selectin
计算分子量83 kDa
观测分子量140 kDa
GenBank蛋白编号NM_011347.2
基因名称 Selp
Gene ID (NCBI) 20344
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ01102
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

P-selectin, also known as GMP140 or CD62P, is a transmembrane glycoprotein that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is an adhesion molecule involved in the pathogenesis of inflammation, thrombosis, and oncogenesis. P-selectin is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.

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