×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 10222-1-AP (same clone as 10222-1-PBS)

    WB analysis using 10222-1-AP (same clone as 10222-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10222-1-AP (Sam68 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10222-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 10222-1-AP (same clone as 10222-1-PBS)

    IP experiment of HeLa using 10222-1-AP (same clone as 10222-1-PBS)

    IP result of anti-Sam68 (IP:10222-1-AP, 4ug; Detection:10222-1-AP 1:1000) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 10222-1-PBS in a different storage buffer formulation.

  • WB analysis using 10222-1-AP (same clone as 10222-1-PBS)

    WB analysis using 10222-1-AP (same clone as 10222-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10222-1-AP (Sam68 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10222-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 10222-1-AP (same clone as 10222-1-PBS)

    IHC staining of mouse colon using 10222-1-AP (same clone as 10222-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10222-1-AP (Sam68 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10222-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KHDRBS1 antibody included
  • IHC staining of mouse colon using 10222-1-AP (same clone as 10222-1-PBS)

    IHC staining of mouse colon using 10222-1-AP (same clone as 10222-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10222-1-AP (Sam68 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10222-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KHDRBS1 antibody included
  • IHC staining of mouse colon using 10222-1-AP (same clone as 10222-1-PBS)

    IHC staining of mouse colon using 10222-1-AP (same clone as 10222-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10222-1-AP (Sam68 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10222-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KHDRBS1 antibody included
  • IF Staining of HepG2 using 10222-1-AP (same clone as 10222-1-PBS)

    IF Staining of HepG2 using 10222-1-AP (same clone as 10222-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Sam68 antibody (10222-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 10222-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Sam68 Polyclonal antibody, PBS Only

Sam68 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 10222-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

KHDRBS1, GAP-associated tyrosine phosphoprotein p62, KH domain-containing, RNA-binding, signal transduction-associated protein 1, KHDRBS 1, p21 Ras GTPase-activating protein-associated p62

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy KHDRBS1 Ready-To-Use IHC Kit. KHDRBS1 primary antibody included.

产品信息

10222-1-PBS targets Sam68 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0235

Product name: Recombinant human Sam68 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 201-442 aa of BC000717

Sequence: GSMRDKAKEEELRKGGDPKYAHLNMDLHVFIEVFGPPCEAYALMAHAMEEVKKFLVPDMMDDICQEQFLELSYLNGVPEPSRGRGVPVRGRGAAPPPPPVPRGRGVGPPRGALVRGTPVRGAITRGATVTRGVPPPPTVRGAPAPRARTAGIQRIPLPPPPAPETYEEYGYDDTYAEQSYEGYEGYYSQSQGDSEYYDYGHGEVQDSYEAYGQDDWNGTRPSLKAPPARPVKGAYREHPYGR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 KH domain containing, RNA binding, signal transduction associated 1
别名 KHDRBS1, GAP-associated tyrosine phosphoprotein p62, KH domain-containing, RNA-binding, signal transduction-associated protein 1, KHDRBS 1, p21 Ras GTPase-activating protein-associated p62
计算分子量 48 kDa
观测分子量 68 kDa
GenBank蛋白编号BC000717
基因名称 Sam68
Gene ID (NCBI) 10657
RRIDAB_2130292
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ07666
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

KHDRBS1, also named as SAM68, p62and p68, belongs to the KHDRBS family. It is recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and INS receptors. Once phosphorylated, KHDRBS1 functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins. It represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. KHDRBS1 also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export. KHDRBS1 has three isoforms with calculated MW of 44-48kd. For isoform with a calculated MW of 48.2 kD, it migrated as a 68kD protein on SDS-PAGE gels[PMID: 10564820]. This ia a rabbit polyclonal antibody raised against residues near the N terminus of human Sam68.

||
New chat

Able™

正在加载，请稍候...