验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse pancreas tissue, U2OS cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12128-1-AP targets SYTL4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, rat, hamster |
| 免疫原 |
CatNo: Ag2772 Product name: Recombinant human SYTL4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 338-671 aa of BC014913 Sequence: IGSMMSIYSEAGDFGNIFVTGRIAFSLKYEQQTQSLVVHVKECHQLAYADEAKKRSNPYVKTYLLPDKSRQGKRKTSIKRDTVNPLYDETLRYEIPESLLAQRTLQFSVWHHGRFGRNTFLGEAEIQMDSWKLDKKLDHCLPLHGKISAESPTGLPSHKGELVVSLKYIPASKTPVGGDRKKSKGGEGGELQVWIKEAKNLTAAKAGGTSDSFVKGYLLPMRNKASKRKTPVMKKTLNPHYNHTFVYNGVRLEDLQHMCLELTVWDREPLASNDFLGGVRLGVGTGISNGEVVDWMDSTGEEVSLWQKMRQYPGSWAEGTLQLRSSMAKQKLGL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | synaptotagmin-like 4 |
| 别名 | Exophilin 2, Exophilin-2, Granuphilin, SLP4, synaptotagmin like 4 |
| 计算分子量 | 671 aa, 76 kDa |
| 观测分子量 | 76 kDa |
| GenBank蛋白编号 | BC014913 |
| 基因名称 | SYTL4 |
| Gene ID (NCBI) | 94121 |
| RRID | AB_2877828 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96C24 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SYTL4, also known as SLP4 or granuphilin, is a member of the synaptotagmin-like protein (Slp) family. Members of this family are characterized by presence of an N-terminal Slp homology domain (SHD), which functions as a Rab27-binding domain, and C-terminal tandem C2 domains, putative Ca(2+)-binding motifs. SYTL4 is specifically expressed on dense-core granules in certain neuroendocrine cells and negatively controls dense-core vesicle exocytosis through specific interaction with Rab27A. (PMID: 16481396; 16473610)
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for SYTL4 antibody 12128-1-AP | Download protocol |
| IP protocol for SYTL4 antibody 12128-1-AP | Download protocol |
| WB protocol for SYTL4 antibody 12128-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Arterioscler Thromb Vasc Biol Kinesin-1: A New Actor Involved in Platelet Secretion and Thrombus Stability. | ||
Theranostics SYTL4 downregulates microtubule stability and confers paclitaxel resistance in triple-negative breast cancer.
| ||
J Proteome Res Identification of tyrosine-phosphorylated proteins associated with lung cancer metastasis using label-free quantitative analyses. | ||
Cell Commun Signal Nesfatin-1 enhances vascular smooth muscle calcification through facilitating BMP-2 osteogenic signaling
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