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验证数据展示

  • WB analysis using 83923-1-RR (same clone as 83923-1-PBS)

    WB analysis using 83923-1-RR (same clone as 83923-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83923-1-RR (Synaptotagmin-14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83923-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 83923-1-RR (same clone as 83923-1-PBS)

    IHC staining of mouse cerebellum using 83923-1-RR (same clone as 83923-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83923-1-RR (Synaptotagmin-14 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83923-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 83923-1-RR (same clone as 83923-1-PBS)

    IHC staining of mouse cerebellum using 83923-1-RR (same clone as 83923-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83923-1-RR (Synaptotagmin-14 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83923-1-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 83923-1-RR (same clone as 83923-1-PBS)

    FC experiment of A431 using 83923-1-RR (same clone as 83923-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Synaptotagmin-14 Recombinant antibody (83923-1-RR, Clone:240866C2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83923-1-PBS in a different storage buffer formulation.

Synaptotagmin-14 Recombinant monoclonal antibody, PBS Only

Synaptotagmin-14 Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83923-1-PBS

产品说明书

CloneNo. 240866C2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), ELISA

别名

240866C2, Synaptotagmin XIV, SYT14, SytXIV

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83923-1-PBS targets Synaptotagmin-14 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 synaptotagmin XIV
别名 240866C2, Synaptotagmin XIV, SYT14, SytXIV
计算分子量 62 kDa
观测分子量62 kDa
GenBank蛋白编号NM_153262
基因名称 Synaptotagmin-14
Gene ID (NCBI) 255928
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ8NB59
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). Synaptotagmin-14 (SYT14) is highly expressed in brain tissues. A homozygous mutation of SYT14 is involved in the pathogenesis of spinocerebellar ataxia (SCA) (PMID: 21835308).

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