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验证数据展示

  • IHC staining of human kidney using 80721-1-RR

    IHC staining of human kidney using 80721-1-RR

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SYNPO antibody included
  • WB analysis using 80721-1-RR

    WB analysis using 80721-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80721-1-RR (SYNPO antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 80721-1-RR

    IHC staining of human kidney using 80721-1-RR

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SYNPO antibody included
  • IHC staining of rat kidney using 80721-1-RR

    IHC staining of rat kidney using 80721-1-RR

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SYNPO antibody included
  • IHC staining of rat kidney using 80721-1-RR

    IHC staining of rat kidney using 80721-1-RR

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SYNPO antibody included
  • IHC staining of mouse kidney using 80721-1-RR

    IHC staining of mouse kidney using 80721-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SYNPO antibody included
  • IHC staining of mouse kidney using 80721-1-RR

    IHC staining of mouse kidney using 80721-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SYNPO antibody included
  • Affinity and Kinetic Characterization of 80721-1-RR

    Affinity and Kinetic Characterization of 80721-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80721-1-RR against Human SYNPO were performed. The affinity constant is 1.38 nM.

SYNPO Recombinant antibody

SYNPO Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 80721-1-RR

产品说明书

CloneNo. 4K5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, ELISA

别名

Synaptopodin, snaptopodin, KIAA1029, 4K5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue, pig brain tissue
Positive IHC detected inhuman kidney tissue, mouse brain tissue, mouse kidney tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SYNPO Ready-To-Use IHC Kit. SYNPO primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:17800
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80721-1-RR targets SYNPO in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 SYNPO fusion protein Ag31287 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 synaptopodin
别名 Synaptopodin, snaptopodin, KIAA1029, 4K5
计算分子量 929 aa, 99 kDa
观测分子量100 kDa
GenBank蛋白编号BC146665
基因名称 SYNPO
Gene ID (NCBI) 11346
RRIDAB_2918907
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8N3V7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Synaptopodin is a actin-associated protein expressed in mature dendritic spines and renal podocytes. Several isoforms of synaptopodin have been reported: the 100 kDa neural form and 110 kDa renal form. 44-45 kDa may represent the degradation product of synaptopodin. As a podocyte specific protein, synaptopodin expression level was decreased in kidney but increased in the urinary podocytes from patients with preeclampsia, indicating that synaptopodin is a useful marker for preeclampsia. (15841212, 10555072)

实验方案

Product Specific Protocols
WB protocol for SYNPO antibody 80721-1-RRDownload protocol
IHC protocol for SYNPO antibody 80721-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols