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验证数据展示

  • IHC staining of human kidney using 80721-1-RR (same clone as 80721-1-PBS)

    IHC staining of human kidney using 80721-1-RR (same clone as 80721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80721-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SYNPO antibody included
  • WB analysis using 80721-1-RR (same clone as 80721-1-PBS)

    WB analysis using 80721-1-RR (same clone as 80721-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80721-1-RR (SYNPO antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80721-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 80721-1-RR (same clone as 80721-1-PBS)

    IHC staining of human kidney using 80721-1-RR (same clone as 80721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80721-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SYNPO antibody included
  • IHC staining of rat kidney using 80721-1-RR (same clone as 80721-1-PBS)

    IHC staining of rat kidney using 80721-1-RR (same clone as 80721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80721-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SYNPO antibody included
  • IHC staining of rat kidney using 80721-1-RR (same clone as 80721-1-PBS)

    IHC staining of rat kidney using 80721-1-RR (same clone as 80721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80721-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SYNPO antibody included
  • IHC staining of mouse kidney using 80721-1-RR (same clone as 80721-1-PBS)

    IHC staining of mouse kidney using 80721-1-RR (same clone as 80721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80721-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SYNPO antibody included
  • IHC staining of mouse kidney using 80721-1-RR (same clone as 80721-1-PBS)

    IHC staining of mouse kidney using 80721-1-RR (same clone as 80721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80721-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SYNPO antibody included
  • Affinity and Kinetic Characterization of 80721-1-RR

    Affinity and Kinetic Characterization of 80721-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80721-1-RR against Human SYNPO were performed. The affinity constant is 1.38 nM.

SYNPO Recombinant antibody, PBS Only

SYNPO Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 80721-1-PBS

产品说明书

CloneNo. 4K5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, Indirect ELISA

别名

Synaptopodin, snaptopodin, KIAA1029, 4K5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SYNPO Ready-To-Use IHC Kit. SYNPO primary antibody included.

产品信息

80721-1-PBS targets SYNPO in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 SYNPO fusion protein Ag31287 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 synaptopodin
别名 Synaptopodin, snaptopodin, KIAA1029, 4K5
计算分子量 929 aa, 99 kDa
观测分子量100 kDa
GenBank蛋白编号BC146665
基因名称 SYNPO
Gene ID (NCBI) 11346
RRIDAB_2918907
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8N3V7
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Synaptopodin is a actin-associated protein expressed in mature dendritic spines and renal podocytes. Several isoforms of synaptopodin have been reported: the 100 kDa neural form and 110 kDa renal form. 44-45 kDa may represent the degradation product of synaptopodin. As a podocyte specific protein, synaptopodin expression level was decreased in kidney but increased in the urinary podocytes from patients with preeclampsia, indicating that synaptopodin is a useful marker for preeclampsia. (15841212, 10555072)