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验证数据展示

  • WB analysis using 29662-1-AP

    WB analysis using 29662-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29662-1-AP (SYNPO antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 29662-1-AP

    IF Staining of U2OS using 29662-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using SYNPO antibody (29662-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of U2OS using 29662-1-AP

    IF Staining of U2OS using 29662-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using SYNPO antibody (29662-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

SYNPO Polyclonal antibody

SYNPO Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 29662-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

KIAA1029, synaptopodin, SYNPO

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IF/ICC detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy SYNPO Ready-To-Use IHC Kit. SYNPO primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

29662-1-AP targets SYNPO in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 Human, mouse, rat
文献引用反应性mouse
免疫原 SYNPO fusion protein Ag31287 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 synaptopodin
别名 KIAA1029, synaptopodin, SYNPO
计算分子量 929 aa, 99 kDa
观测分子量44 kDa, 74-100 kDa
GenBank蛋白编号BC146665
基因名称 SYNPO
Gene ID (NCBI) 11346
RRIDAB_3086150
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N3V7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Synaptopodin is a actin-associated protein expressed in mature dendritic spines and renal podocytes. Several isoforms of synaptopodin have been reported: the 100 kDa neural form and 110 kDa renal form. 44-45 kDa may represent the degradation product of synaptopodin. As a podocyte specific protein, synaptopodin expression level was decreased in kidney but increased in the urinary podocytes from patients with preeclampsia, indicating that synaptopodin is a useful marker for preeclampsia. (15841212, 10555072)

实验方案

Product Specific Protocols
WB protocol for SYNPO antibody 29662-1-APDownload protocol
IF protocol for SYNPO antibody 29662-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Arch Physiol Biochem

Huaiqihuang (HQH) protects podocytes from high glucose-induced apoptosis and inflammation response by regulating PI3K/AKT/mTOR pathway

Authors - Peipei Zhang
View Article