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SYNPO Polyclonal antibody

SYNPO Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 29662-1-AP

Print datasheet

Synonyms

KIAA1029, synaptopodin, SYNPO

验证数据展示

  • WB analysis using 29662-1-AP

    WB analysis using 29662-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29662-1-AP (SYNPO antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 29662-1-AP

    IF Staining of U2OS using 29662-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using SYNPO antibody (29662-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of U2OS using 29662-1-AP

    IF Staining of U2OS using 29662-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using SYNPO antibody (29662-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IF detected inU2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29662-1-AP targets SYNPO in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen SYNPO fusion protein Ag31287 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name synaptopodin
Synonyms KIAA1029, synaptopodin, SYNPO
Calculated Molecular Weight 929 aa, 99 kDa
Observed Molecular Weight44 kDa, 74-100 kDa
GenBank Accession NumberBC146665
Gene Symbol SYNPO
Gene ID (NCBI) 11346
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N3V7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Synaptopodin is a actin-associated protein expressed in mature dendritic spines and renal podocytes. Several isoforms of synaptopodin have been reported: the 100 kDa neural form and 110 kDa renal form. 44-45 kDa may represent the degradation product of synaptopodin. As a podocyte specific protein, synaptopodin expression level was decreased in kidney but increased in the urinary podocytes from patients with preeclampsia, indicating that synaptopodin is a useful marker for preeclampsia. (15841212, 10555072)

实验方案

Product Specific Protocols
WB protocol for SYNPO antibody 29662-1-APDownload protocol
IF protocol for SYNPO antibody 29662-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols