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验证数据展示

  • WB analysis of mouse brain using 24677-1-AP (same clone as 24677-1-PBS)

    WB analysis of mouse brain using 24677-1-AP (same clone as 24677-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24677-1-AP (SYNJ1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24677-1-PBS in a different storage buffer formulation.

  • WB analysis using 24677-1-AP (same clone as 24677-1-PBS)

    WB analysis using 24677-1-AP (same clone as 24677-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24677-1-AP (SYNJ1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24677-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 24677-1-AP (same clone as 24677-1-PBS)

    IHC staining of human brain using 24677-1-AP (same clone as 24677-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24677-1-AP (SYNJ1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 24677-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 24677-1-AP (same clone as 24677-1-PBS)

    IHC staining of human brain using 24677-1-AP (same clone as 24677-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24677-1-AP (SYNJ1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 24677-1-PBS in a different storage buffer formulation.

  • IHC staining of rat skeletal muscle using 24677-1-AP (same clone as 24677-1-PBS)

    IHC staining of rat skeletal muscle using 24677-1-AP (same clone as 24677-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 24677-1-AP (SYNJ1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24677-1-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 24677-1-AP (same clone as 24677-1-PBS)

    IF Staining of NIH/3T3 using 24677-1-AP (same clone as 24677-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 24677-1-AP (SYNJ1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24677-1-PBS in a different storage buffer formulation.

  • IHC staining of rat skeletal muscle using 24677-1-AP (same clone as 24677-1-PBS)

    IHC staining of rat skeletal muscle using 24677-1-AP (same clone as 24677-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 24677-1-AP (SYNJ1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24677-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 24677-1-AP (same clone as 24677-1-PBS)

    IHC staining of rat brain using 24677-1-AP (same clone as 24677-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24677-1-AP (SYNJ1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24677-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 24677-1-AP (same clone as 24677-1-PBS)

    IHC staining of rat brain using 24677-1-AP (same clone as 24677-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24677-1-AP (SYNJ1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24677-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 24677-1-AP (same clone as 24677-1-PBS)

    IP experiment of mouse brain using 24677-1-AP (same clone as 24677-1-PBS)

    IP result of anti-SYNJ1 (IP:24677-1-AP, 4ug; Detection:24677-1-AP 1:5000) with mouse brain tissue lysate 1960 ug. This data was developed using the same antibody clone with 24677-1-PBS in a different storage buffer formulation.

SYNJ1 Polyclonal antibody, PBS Only

SYNJ1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 24677-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

EC:3.1.3.36, INPP5G, KIAA0910, Synaptic inositol 1,4,5-trisphosphate 5-phosphatase 1, synaptojanin 1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

24677-1-PBS targets SYNJ1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag20364

Product name: Recombinant human SYNJ1 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 856-1125 aa of BC136603

Sequence: PVVALIDIDIFEVEAEERQNIYKEVIAVQGPPDGTVLVSIKSSLPENNFFDDALIDELLQQFASFGEVILIRFVEDKMWVTFLEGSSALNVLSLNGKELLNRTITIALKSPDWIKNLEEEMSLEKISIALPSSTSSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTISEGPVPSLPIRPSRAPSRTPGPPSAQSSPIDAQPATPLPQKDPAQPLEPKRPPPPRPVAPPTRPAPPQRPPPPS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 synaptojanin 1
别名 EC:3.1.3.36, INPP5G, KIAA0910, Synaptic inositol 1,4,5-trisphosphate 5-phosphatase 1, synaptojanin 1
计算分子量 1612 aa, 178 kDa
观测分子量 140 kDa
GenBank蛋白编号BC136603
基因名称 SYNJ1
Gene ID (NCBI) 8867
RRIDAB_2879668
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43426
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Synaptojanin 1, a polyphosphoinositide phosphatase, is expressed as two major alternatively spliced isoforms of 145 kDa (SJ145) and 170 kDa (SJ170), which are thought to have pleiotropic roles in endocytosis, signaling, and actin function. SJ145 is highly enriched in nerve terminals where it participates in clathrin-dependent synaptic vesicle recycling. SJ170, which differs from SJ145 by the presence of a carboxy-terminal extension, is the predominant isoform in developing neurons and is expressed in a variety of tissues (PMID:10801423).

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