验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, NIH/3T3 cells, rat brain tissue |
| Positive IHC detected in | human brain tissue, human skeletal muscle tissue, rat brain tissue, rat skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24677-1-AP targets SYNJ1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IP, IHC, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | SYNJ1 fusion protein Ag20364 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | synaptojanin 1 |
| 别名 | INPP5G, KIAA0910, synaptojanin 1, SYNJ1 |
| 计算分子量 | 1612 aa, 178 kDa |
| 观测分子量 | 140 kDa |
| GenBank蛋白编号 | BC136603 |
| 基因名称 | SYNJ1 |
| Gene ID (NCBI) | 8867 |
| RRID | AB_2879668 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O43426 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Synaptojanin 1, a polyphosphoinositide phosphatase, is expressed as two major alternatively spliced isoforms of 145 kDa (SJ145) and 170 kDa (SJ170), which are thought to have pleiotropic roles in endocytosis, signaling and actin function. SJ145 is highly enriched in nerve terminals where it participates in clathrin-dependent synaptic vesicle recycling. SJ170, which differs from SJ145 by the presence of a carboxy-terminal extension, is the predominant isoform in developing neurons and is expressed in a variety of tissues.(PMID:10801423).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SYNJ1 antibody 24677-1-AP | Download protocol |
| IHC protocol for SYNJ1 antibody 24677-1-AP | Download protocol |
| IF protocol for SYNJ1 antibody 24677-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Chem Biol Triplexed Affinity Reagents to Sample the Mammalian Inositol Pyrophosphate Interactome. | ||
Behav Brain Res SYNJ1 rescues motor functions in hereditary and sporadic Parkinson's disease mice by upregulating TSP-1 expression | ||
Ren Fail Network pharmacology and molecular docking of endogenous active metabolites in diabetic kidney disease | ||
Cell Signal Identification of novel PCTAIRE-1/CDK16 substrates using a chemical genetic screen. |
