验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells |
| Positive IF detected in | Human dorsal cerebral organoid, mouse brain tissue, rat brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
25265-1-AP targets Nesprin 2 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | spectrin repeat containing, nuclear envelope 2 |
| 别名 | SYNE2, SYNE 2, NUANCE, NUA |
| 计算分子量 | 799 kDa |
| 观测分子量 | 50-60 kDa |
| GenBank蛋白编号 | NM_015180 |
| 基因名称 | Nesprin 2 |
| Gene ID (NCBI) | 23224 |
| RRID | AB_2879995 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8WXH0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Nesprin-2 is a member of actin-binding proteins that localize to the outer nuclear membrane. As a nuclear envelope component it provides a link between cytoskeletal components of the cytoplasm and the nucleoplasm. Nesprin genes generate, through alternative splicing and alternative promoter and/or terminator usage, a multitude of differentially expressed isoforms, differing in domain architecture and length. The largest protein isoform, known as NUANCE or GIANT, has a predicted MW around 796 kDa. Other isoforms range from 30 kDa to 119 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Nesprin 2 antibody 25265-1-AP | Download protocol |
| WB protocol for Nesprin 2 antibody 25265-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
