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验证数据展示

  • WB analysis using 30549-1-AP

    WB analysis using 30549-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30549-1-AP (SYN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse pancreas using 30549-1-AP

    IHC staining of mouse pancreas using 30549-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 30549-1-AP (SYN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SYN1 Polyclonal antibody

SYN1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30549-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Synapsin I, Brain protein 4.1, SYN1a, SYN1b, Synapsin 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inIMR-32 cells, SH-SY5Y cells, SK-N-SH cells
Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

30549-1-AP targets SYN1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原 SYN1 fusion protein Ag31091 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 synapsin I
别名 Synapsin I, Brain protein 4.1, SYN1a, SYN1b, Synapsin 1
计算分子量 74 kDa
观测分子量70-80 kDa
GenBank蛋白编号NM_006950
基因名称 SYN1
Gene ID (NCBI) 6853
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP17600
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SYN1, also named Synapsin I, Brain protein 4.1, belongs to the Synapsin family. SYN1 is a neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to regulate neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level. Defects in SYN1 are a cause of epilepsy X-linked with variable learning disabilities and behavior disorders (XELBD). Synapsin I (SYN1) is composed of two isoforms, synapsin Ia and Ib, with molecular weights of 74 kDa and 70 kDa, respectively.

实验方案

Product Specific Protocols
WB protocol for SYN1 antibody 30549-1-APDownload protocol
IHC protocol for SYN1 antibody 30549-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Redox Biol

Design of anti-depressant phosphodiester amino acidic Keap1-Nrf2 protein-protein interaction inhibitors

Authors - Yi Sun
View Article