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验证数据展示

  • WB analysis using 30549-1-AP

    WB analysis using 30549-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30549-1-AP (SYN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse pancreas using 30549-1-AP

    IHC staining of mouse pancreas using 30549-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 30549-1-AP (SYN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SYN1 Polyclonal antibody

SYN1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30549-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Synapsin I, Brain protein 4.1, SYN1a, SYN1b, Synapsin 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inIMR-32 cells, SH-SY5Y cells, SK-N-SH cells
Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30549-1-AP targets SYN1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 SYN1 fusion protein Ag31091 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 synapsin I
别名 Synapsin I, Brain protein 4.1, SYN1a, SYN1b, Synapsin 1
计算分子量 74 kDa
观测分子量70-80 kDa
GenBank蛋白编号NM_006950
基因名称 SYN1
Gene ID (NCBI) 6853
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP17600
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SYN1, also named Synapsin I, Brain protein 4.1, belongs to the Synapsin family. SYN1 is a neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to regulate neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level. Defects in SYN1 are a cause of epilepsy X-linked with variable learning disabilities and behavior disorders (XELBD). Synapsin I (SYN1) is composed of two isoforms, synapsin Ia and Ib, with molecular weights of 74 kDa and 70 kDa, respectively.

实验方案

Product Specific Protocols
WB protocol for SYN1 antibody 30549-1-APDownload protocol
IHC protocol for SYN1 antibody 30549-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols