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验证数据展示

  • WB analysis using 31030-1-AP (same clone as 31030-1-PBS)

    WB analysis using 31030-1-AP (same clone as 31030-1-PBS)

    Various lysates were subjected to Tris-acetate followed by western blot with 31030-1-AP (SVEP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31030-1-PBS in a different storage buffer formulation.

  • WB analysis using 31030-1-AP (same clone as 31030-1-PBS)

    WB analysis using 31030-1-AP (same clone as 31030-1-PBS)

    Various lysates were subjected to Tris-Acetate gel system followed by western blot with 31030-1-AP (SVEP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31030-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 31030-1-AP (same clone as 31030-1-PBS)

    IHC staining of human placenta using 31030-1-AP (same clone as 31030-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 31030-1-AP (SVEP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31030-1-PBS in a different storage buffer formulation.

SVEP1 Polyclonal antibody, PBS Only

SVEP1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 31030-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

C9orf13, CCP22, POLYDOM, SEL OB, SELOB

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

31030-1-PBS targets SVEP1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag34316

Product name: Recombinant human SVEP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 736-900 aa of NM_153366

Sequence: GDFICTPDNTGVNCTLTCLEGYDFTEGSTDKYYCAYEDGVWKPTYTTEWPDCAKKRFANHGFKSFEMFYKAARCDDTDLMKKFSEAFETTLGKMVPSFCSDAEDIDCRLEENLTKKYCLEYNYDYENGFAIGPGGWGAANRLDYSYDDFLDTVQETATSIGNAKS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sushi, von Willebrand factor type A, EGF and pentraxin domain containing 1
别名 C9orf13, CCP22, POLYDOM, SEL OB, SELOB
计算分子量390 kDa
观测分子量460 kDa
GenBank蛋白编号NM_153366
基因名称 SVEP1
Gene ID (NCBI) 79987
RRIDAB_3742391
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ4LDE5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SVEP1 (C9orf13), which is predicted to be located in the cell membrane, nucleus and cytoplasm. The protein is expressed in the whole epidermis, especially in the basal layer of epidermis, the upper and lower layers of basal layer and dermal fibroblasts in the upper dermis (PMID:27892606). At the same time, it is highly expressed in placenta, lung, adrenal gland and cerebellum, but weakly expressed in heart and skeletal muscle (PMID:11062057, PMID:27892606). The molecular weight of SVEP1 is 390 kDa. At the same time, it also has several isomers of 96kDa, 163kDa and 170kDa, and there are also glycosylation modifications. It is reported that the molecular weight is 460 kDa (PMID: 32371982). The protein may plays a role in initial cell attachment of stromal osteogenic cells.

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