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验证数据展示

  • WB analysis using 15379-1-AP (same clone as 15379-1-PBS)

    WB analysis using 15379-1-AP (same clone as 15379-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15379-1-AP (SURF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15379-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 15379-1-AP (same clone as 15379-1-PBS)

    IF Staining of HeLa using 15379-1-AP (same clone as 15379-1-PBS)

    Immunofluorescent analysis of HeLa cells using 15379-1-AP (SURF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15379-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 15379-1-AP (same clone as 15379-1-PBS)

    IP experiment of HeLa using 15379-1-AP (same clone as 15379-1-PBS)

    IP result of anti-SURF1 (IP:15379-1-AP, 4ug; Detection:15379-1-AP 1:2000) with HeLa cells lysate 1575 ug. This data was developed using the same antibody clone with 15379-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 15379-1-AP (same clone as 15379-1-PBS)

    IHC staining of human colon using 15379-1-AP (same clone as 15379-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15379-1-AP (SURF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15379-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 15379-1-AP (same clone as 15379-1-PBS)

    IHC staining of human colon using 15379-1-AP (same clone as 15379-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15379-1-AP (SURF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15379-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SURF1 Polyclonal antibody, PBS Only

SURF1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 15379-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

SURF 1, SURF-1, surfeit 1, Surfeit locus protein 1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

15379-1-PBS targets SURF1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag3959

Product name: Recombinant human SURF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-300 aa of BC028314

Sequence: MAAVAALQLGLRAAGLGRAPASAAWRSVLRVSPRPGVAWRPSRCGSSAAEASATKAEDDSFLQWVLLLIPVTAFGLGTWQVQRRKWKLNLIAELESRVLAEPVPLPADPMELKNLEYRPVKVRGCFDHSKELYMMPRTMVDPVREAREGGLISSSTQSGAYVVTPFHCTDLGVTILVNRGFVPRKKVNPETRQKGQIEGEVDLIGMVRLTETRQPFVPENNPERNHWHYRDLEAMARITGAEPIFIDANFQSTVPGGPIGGQTRVTLRNEHLQYIVTWYGLSAATSYLWFKKFLRGTPGV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 surfeit 1
别名 SURF 1, SURF-1, surfeit 1, Surfeit locus protein 1
计算分子量 33 kDa
观测分子量 30-31 kDa
GenBank蛋白编号BC028314
基因名称 SURF1
Gene ID (NCBI) 6834
RRIDAB_2239968
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15526
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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