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验证数据展示

  • WB analysis using 86706-1-RR (same clone as 86706-1-PBS)

    WB analysis using 86706-1-RR (same clone as 86706-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86706-1-RR (SUPV3L1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86706-1-PBS in a different storage buffer formulation.

  • WB analysis of rat kidney using 86706-1-RR (same clone as 86706-1-PBS)

    WB analysis of rat kidney using 86706-1-RR (same clone as 86706-1-PBS)

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 86706-1-RR (SUPV3L1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86706-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 86706-1-RR (same clone as 86706-1-PBS)

    IHC staining of human liver cancer using 86706-1-RR (same clone as 86706-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 86706-1-RR (SUPV3L1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86706-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 86706-1-RR (same clone as 86706-1-PBS)

    IF Staining of HeLa using 86706-1-RR (same clone as 86706-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SUPV3L1 antibody (86706-1-RR, Clone: 250855G8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86706-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP02602-1

    Cytometric bead array sample test of MP02602-1

    The mean SUPV3L1 concentration was determined to be 102.3 ng/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load, 171.2 ng/mL in PC-3 cell extract based on a 1.3 mg/mL extract load.

  • Cytometric bead array standard curve of MP02602-1

    Cytometric bead array standard curve of MP02602-1

    Cytometric bead array standard curve of MP02602-1, SUPV3L1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86706-1-PBS. Detection antibody: 86706-3-PBS. Standard: Ag3542. Range: 1.562-200 ng/mL.

SUPV3L1 Recombinant monoclonal antibody, PBS Only (Capture)

SUPV3L1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 86706-1-PBS

产品说明书

CloneNo. 250855G8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

ATP-dependent RNA helicase SUPV3L1, mitochondrial, EC:3.6.4.13, Suppressor of var1 3-like protein 1, SUV3, SUV3 like protein 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86706-1-PBS targets SUPV3L1 as part of a matched antibody pair:

MP02602-1: 86706-1-PBS capture and 86706-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3542

Product name: Recombinant human SUPV3L1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 441-786 aa of BC036112

Sequence: YSLIKPSINEKGERELEPITTSQALQIAGRAGRFSSRFKEGEVTTMNHEDLSLLKEILKRPVDPIRAAGLHPTAEQIEMFAYHLPDATLSNLIDIFVDFSQVDGQYFVCNMDDFKFSAELIQHIPLSLRVRYVFCTAPINKKQPFVCSSLLQFARQYSRNEPLTFAWLRRYIKWPLLPPKNIKDLMDLEAVHDVLDLYLWLSYRFMDMFPDASLIRDLQKELDGIIQDGVHNITKLIKMSETHKLLNLEGFPSGSQSRLSGTLKSQARRTRGTKALGSKATEPPSPDAGELSLASRLVQQGLLTPDMLKQLEKEWMTQQTEHNKEKTESGTHPKGTRRKKKEPDSD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 suppressor of var1, 3-like 1 (S. cerevisiae)
别名 ATP-dependent RNA helicase SUPV3L1, mitochondrial, EC:3.6.4.13, Suppressor of var1 3-like protein 1, SUV3, SUV3 like protein 1
计算分子量 786 aa, 88 kDa
观测分子量 70-80 kDa
GenBank蛋白编号BC036112
基因名称 SUPV3L1
Gene ID (NCBI) 6832
RRIDAB_3745068
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8IYB8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SUPV3L1 is a major helicase player in mitochondrial RNA metabolism. It is involved in the degradation of non-coding mitochondrial transcripts (MT-ncRNA) and tRNA-like molecules (PMID: 29967381).

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