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验证数据展示

  • IP experiment of HeLa using 12826-1-AP

    IP experiment of HeLa using 12826-1-AP

    IP result of anti-SUPV3L1 (IP:12826-1-AP, 4ug; Detection:12826-1-AP 1:300) with HeLa cells lysate 1200ug.

  • IF Staining of HeLa using 12826-1-AP

    IF Staining of HeLa using 12826-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 12826-1-AP (SUPV3L1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 12826-1-AP

    WB analysis using 12826-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12826-1-AP (SUPV3L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis using 12826-1-AP

    WB analysis using 12826-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12826-1-AP (SUPV3L1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 12826-1-AP

    IHC staining of human stomach cancer using 12826-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12826-1-AP (SUPV3L1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SUPV3L1 Polyclonal antibody

SUPV3L1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 12826-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

SUV3-like protein 1, SUV3 like protein 1, SUV3, Suppressor of var1 3-like protein 1, EC:3.6.4.13

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, PC-3 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

12826-1-AP targets SUPV3L1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag3542

Product name: Recombinant human SUPV3L1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 441-786 aa of BC036112

Sequence: YSLIKPSINEKGERELEPITTSQALQIAGRAGRFSSRFKEGEVTTMNHEDLSLLKEILKRPVDPIRAAGLHPTAEQIEMFAYHLPDATLSNLIDIFVDFSQVDGQYFVCNMDDFKFSAELIQHIPLSLRVRYVFCTAPINKKQPFVCSSLLQFARQYSRNEPLTFAWLRRYIKWPLLPPKNIKDLMDLEAVHDVLDLYLWLSYRFMDMFPDASLIRDLQKELDGIIQDGVHNITKLIKMSETHKLLNLEGFPSGSQSRLSGTLKSQARRTRGTKALGSKATEPPSPDAGELSLASRLVQQGLLTPDMLKQLEKEWMTQQTEHNKEKTESGTHPKGTRRKKKEPDSD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 suppressor of var1, 3-like 1 (S. cerevisiae)
别名 SUV3-like protein 1, SUV3 like protein 1, SUV3, Suppressor of var1 3-like protein 1, EC:3.6.4.13
计算分子量 786 aa, 88 kDa
观测分子量 70-80 kDa
GenBank蛋白编号BC036112
基因名称 SUPV3L1
Gene ID (NCBI) 6832
RRIDAB_2877885
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IYB8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SUPV3L1 is a major helicase player in mitochondrial RNA metabolism. It is involved in the degradation of non-coding mitochondrial transcripts (MT-ncRNA) and tRNA-like molecules (PMID: 29967381).

实验方案

Product Specific Protocols
WB protocol for SUPV3L1 antibody 12826-1-APDownload protocol
IHC protocol for SUPV3L1 antibody 12826-1-APDownload protocol
IF protocol for SUPV3L1 antibody 12826-1-APDownload protocol
IP protocol for SUPV3L1 antibody 12826-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Inherit Metab Dis

Mitochondrial RNA processing defect caused by a SUPV3L1 mutation in two siblings with a novel neurodegenerative syndrome.

Authors - Selma L van Esveld
View Article
humanWB

Biochimie

Cathepsin D inhibition during neuronal differentiation selectively affects individual proteins instead of overall protein turnover

Authors - Johannes Schneider
View Article