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验证数据展示

  • WB analysis of HeLa using 23073-1-AP (same clone as 23073-1-PBS)

    WB analysis of HeLa using 23073-1-AP (same clone as 23073-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 23073-1-AP (SUPT6H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23073-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 23073-1-AP (same clone as 23073-1-PBS)

    IP experiment of HEK-293 using 23073-1-AP (same clone as 23073-1-PBS)

    IP result of anti-SUPT6H (IP:23073-1-AP, 4ug; Detection:23073-1-AP 1:1000) with HEK-293 cells lysate 1200ug. This data was developed using the same antibody clone with 23073-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 23073-1-AP (same clone as 23073-1-PBS)

    IHC staining of human stomach cancer using 23073-1-AP (same clone as 23073-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23073-1-AP (SUPT6H antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23073-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SUPT6H antibody included

SUPT6H Polyclonal antibody, PBS Only

SUPT6H Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 23073-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, Indirect ELISA

别名

SPT6H, SPT6, KIAA0162, hSPT6, Histone chaperone suppressor of Ty6

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SUPT6H Ready-To-Use IHC Kit. SUPT6H primary antibody included.

产品信息

23073-1-PBS targets SUPT6H in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag19426

Product name: Recombinant human SUPT6H protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1618-1726 aa of BC136524

Sequence: TPSYSYTTPSQPITTPQYHQLQASTTPQSAQAQPQPSSSSRQRQQQPKSNSHAAIDWGKMAEQWLQEKEAERRKQKQRLTPRPSPSPMIESTPMSIAGDATPLLDEMDR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 suppressor of Ty 6 homolog (S. cerevisiae)
别名 SPT6H, SPT6, KIAA0162, hSPT6, Histone chaperone suppressor of Ty6
计算分子量 1726 aa, 199 kDa
观测分子量 230 kDa
GenBank蛋白编号BC136524
基因名称 SUPT6H
Gene ID (NCBI) 6830
RRIDAB_11232593
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7KZ85
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SUPT6H (suppressor of Ty6 homolog), also known as SPT6, SPT6H, Tat-CT2 (Tat-cotransactivator 2 protein) or emb-5 in C. elegans, is a 1,726 amino acid protein that is highly conserved from yeast to humans. Expressed ubiquitously, SUPT6H localizes to the nucleus and contains one SH2 domain and one S1 domain. SUPT6H participates in both DRB (5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole)-mediated transcriptional inhibition as well as the enhancement of transcriptional elongation by the RNA polymerase II (Pol II). SUPT6H interacts with the nuclear proteins SPT4 and SPT5, which comprise the DSIF (DRB-sensitivity-inducing factor) complex that binds RNA polymerase II, and directly regulates elongation. Via its C-terminus, SUPT6H can also interact with Histone H3. Due to alternative splicing events, three isoforms exist for SUPT6H. This antibody specifically recognizes the 230kd phosphorylated SUPT6H protein.

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