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SUPT6H Polyclonal antibody

SUPT6H Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 23073-1-AP

Print datasheet

Synonyms

emb 5, hSPT6, KIAA0162, SPT6, SPT6H, SUPT6H, Tat cotransactivator 2 protein, Tat CT2 protein

验证数据展示

  • WB analysis of HeLa using 23073-1-AP

    WB analysis of HeLa using 23073-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23073-1-AP (SUPT6H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 23073-1-AP

    IP experiment of HEK-293 using 23073-1-AP

    IP result of anti-SUPT6H (IP:23073-1-AP, 4ug; Detection:23073-1-AP 1:1000) with HEK-293 cells lysate 1200ug.

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells
Positive IP detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy SUPT6H Ready-To-Use IHC Kit. SUPT6H primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 23073-1-AP is SUPT6H Fusion Protein expressed in E. coli.

Tested Applications IP, WB,ELISA
Cited ApplicationsWB, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen SUPT6H fusion protein Ag19426 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name suppressor of Ty 6 homolog (S. cerevisiae)
Synonyms emb 5, hSPT6, KIAA0162, SPT6, SPT6H, SUPT6H, Tat cotransactivator 2 protein, Tat CT2 protein
Calculated Molecular Weight 1726 aa, 199 kDa
Observed Molecular Weight 230 kDa
GenBank Accession NumberBC136524
Gene Symbol SUPT6H
Gene ID (NCBI) 6830
RRIDAB_11232593
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7KZ85
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SUPT6H (suppressor of Ty6 homolog), also known as SPT6, SPT6H, Tat-CT2 (Tat-cotransactivator 2 protein) or emb-5 in C. elegans, is a 1,726 amino acid protein that is highly conserved from yeast to humans. Expressed ubiquitously, SUPT6H localizes to the nucleus and contains one SH2 domain and one S1 domain. SUPT6H participates in both DRB (5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole)-mediated transcriptional inhibition as well as the enhancement of transcriptional elongation by the RNA polymerase II (Pol II). SUPT6H interacts with the nuclear proteins SPT4 and SPT5, which comprise the DSIF (DRB-sensitivity-inducing factor) complex that binds RNA polymerase II, and directly regulates elongation. Via its C-terminus, SUPT6H can also interact with Histone H3. Due to alternative splicing events, three isoforms exist for SUPT6H. This antibody specifically recognizes the 230kd phosphorylated SUPT6H protein.

实验方案

Product Specific Protocols
WB protocol for SUPT6H antibody 23073-1-APDownload protocol
IP protocol for SUPT6H antibody 23073-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

Nat Commun

TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV.

Authors - Roy Matkovic
View Article