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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67559-1-Ig (same clone as 67559-1-PBS)

    WB analysis using 67559-1-Ig (same clone as 67559-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67559-1-Ig (SUMO1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67559-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 67559-1-Ig (same clone as 67559-1-PBS)

    IF Staining of MCF-7 using 67559-1-Ig (same clone as 67559-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SUMO1 antibody (67559-1-Ig, Clone: 3B5B5 ) at dilution of 1:1600 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67559-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 67559-1-Ig (same clone as 67559-1-PBS)

    IHC staining of mouse kidney using 67559-1-Ig (same clone as 67559-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67559-1-Ig (SUMO1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67559-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SUMO1 antibody included
  • IF Staining of A549 using 67559-1-Ig (same clone as 67559-1-PBS)

    IF Staining of A549 using 67559-1-Ig (same clone as 67559-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using SUMO1 antibody (67559-1-Ig, Clone: 3B5B5 ) at dilution of 1:3200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67559-1-PBS in a different storage buffer formulation.

SUMO1 Monoclonal antibody, PBS Only

SUMO1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67559-1-PBS

产品说明书

CloneNo. 3B5B5

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Small ubiquitin related modifier 1, Sentrin, OK/SW-cl.43, GMP1, GAP-modifying protein 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SUMO1 Ready-To-Use IHC Kit. SUMO1 primary antibody included.

产品信息

67559-1-PBS targets SUMO1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SUMO1 fusion protein Ag29402 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)
别名 Small ubiquitin related modifier 1, Sentrin, OK/SW-cl.43, GMP1, GAP-modifying protein 1
计算分子量 12 kDa
观测分子量 15-100 kDa
GenBank蛋白编号BC006462
基因名称 SUMO1
Gene ID (NCBI) 7341
RRIDAB_2882773
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP63165
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ubiquitin is most famous for its function in targeting proteins for degradation by the 26S proteasome, ubiquitin needs to be attached to a substrate in chains (polyubiquitylation) before being recognized by proteasome. Similarly, SUMO (small ubiquitin-related modifier) can be linked to substrates in chains (polysumoylation), SUMO modification has been implicated in many important cellular processes including the control of genome stability, signal transduction, targeting to and formation of nuclear compartments, cell cycle and meiosis. There are 4 confirmed SUMO isoforms in human, SUMO-1, SUMO-2, SUMO-3 and SUMO-4. SUMO-2 and SUMO-3 are nearly identical but are distinct from SUMO-1. SUMO2/3 conjugation was recently widely involved in neuroprotective activities. A substitution (M55V) of SUMO4 was strongly associated with the pathogenesis of type 1 diabetes (T1D) involving NF kappa B related mechanisms.This antibody can detect endogenous levels of SUMOylated proteins (e.g. SUMO-1-RanGAP at 80-90 kD).