• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

SUMF2 Polyclonal antibody

SUMF2 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 11210-1-AP

Print datasheet

Synonyms

pFGE, sulfatase modifying factor 2, SUMF2, UNQ1968/PRO4500

验证数据展示

  • WB analysis using 11210-1-AP

    WB analysis using 11210-1-AP

    A431 cell lysates were subjected to SDS PAGE followed by western blot with 11210-1-AP (SUMF2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 11210-1-AP

    IHC staining of human breast cancer using 11210-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11210-1-AP (SUMF2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 11210-1-AP

    IHC staining of human breast cancer using 11210-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11210-1-AP (SUMF2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of A431 using 11210-1-AP

    IP experiment of A431 using 11210-1-AP

    IP result of anti-SUMF2 (IP:11210-1-AP, 4ug; Detection:11210-1-AP 1:2000) with A431 cells lysate 2640 ug.

  • IF Staining of A431 using 11210-1-AP

    IF Staining of A431 using 11210-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed A431 cells using 11210-1-AP (SUMF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IP experiment of HeLa using 11210-1-AP

    IP experiment of HeLa using 11210-1-AP

    IP result of anti-SUMF2 (IP:11210-1-AP, 4ug; Detection:11210-1-AP 1:2000) with HeLa cells lysate 1560 ug.

经过测试的应用

Positive WB detected inA431 cells, human skin tissue, human spleen tissue
Positive IP detected inA431 cells, HeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11210-1-AP targets SUMF2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityrat
Immunogen SUMF2 fusion protein Ag1711 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sulfatase modifying factor 2
Synonyms pFGE, sulfatase modifying factor 2, SUMF2, UNQ1968/PRO4500
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC015600
Gene Symbol SUMF2
Gene ID (NCBI) 25870
RRIDAB_2255511
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NBJ7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SUMF2 antibody 11210-1-APDownload protocol
IHC protocol for SUMF2 antibody 11210-1-APDownload protocol
IF protocol for SUMF2 antibody 11210-1-APDownload protocol
IP protocol for SUMF2 antibody 11210-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB,IHC

Int J Clin Exp Pathol

Downregulation of SUMF2 gene in ovalbumin-induced rat model of allergic inflammation.

Authors - Chuanfeng Fang
View Article