验证数据展示
经过测试的应用
| Positive WB detected in | fetal human brain tissue, Y79 cells, pig brain tissue, rat brain tissue, mouse brain tissue, pig cerebellum tissue, rat cerebellum tissue, mouse cerebellum tissue |
| Positive IHC detected in | mouse brain tissue, mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive FC (Intra) detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy STXBP1 Ready-To-Use IHC Kit. STXBP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
67137-1-Ig targets STXBP1 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, IHC, IF-P, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat, pig |
| 文献引用反应性 | human |
| 免疫原 | STXBP1 fusion protein Ag28605 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | syntaxin binding protein 1 |
| 别名 | N Sec1, Munc18-1, MUNC18 1, FLJ37475, EIEE4 |
| 计算分子量 | 594 aa, 68 kDa |
| 观测分子量 | 68 kDa |
| GenBank蛋白编号 | BC015749 |
| 基因名称 | STXBP1 |
| Gene ID (NCBI) | 6812 |
| RRID | AB_2882436 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P61764 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
STXBP1, also named as UNC18A, N-Sec1 and p67 belongs to the STXBP/unc-18/SEC1 family. STXBP1 may participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. It is essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. STXBP1 can interact with syntaxins 1, 2, and 3 but not syntaxin 4. It may play a role in determining the specificity of intracellular fusion reactions.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for STXBP1 antibody 67137-1-Ig | Download protocol |
| IHC protocol for STXBP1 antibody 67137-1-Ig | Download protocol |
| IF protocol for STXBP1 antibody 67137-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
