STXBP1 Monoclonal antibody
STXBP1 Monoclonal Antibody for FC, IF-P, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, mouse, rat, pig and More (1)
Applications
FC, IF-P, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
1B5B3
验证数据展示
经过测试的应用
| Positive WB detected in | fetal human brain tissue, Y79 cells, pig brain tissue, rat brain tissue, mouse brain tissue, pig cerebellum tissue, rat cerebellum tissue, mouse cerebellum tissue |
| Positive IHC detected in | mouse brain tissue, mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse brain tissue |
| Positive FC detected in | HeLa cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
67137-1-Ig targets STXBP1 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.
| Tested Applications | FC, IF-P, IHC, WB, ELISA |
| Cited Applications | WB |
| Tested Reactivity | Human, mouse, rat, pig |
| Cited Reactivity | human |
| Immunogen | STXBP1 fusion protein Ag28605 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | syntaxin binding protein 1 |
| Synonyms | EIEE4, FLJ37475, MUNC18 1, N Sec1, p67, Protein unc 18 homolog 1, Protein unc 18 homolog A, RBSEC1, STXBP1, syntaxin binding protein 1, Unc 18A, UNC18, Unc18 1, UNC18A |
| Calculated Molecular Weight | 594 aa, 68 kDa |
| Observed Molecular Weight | 68 kDa |
| GenBank Accession Number | BC015749 |
| Gene Symbol | STXBP1 |
| Gene ID (NCBI) | 6812 |
| RRID | AB_2882436 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P61764 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
STXBP1, also named as UNC18A, N-Sec1 and p67 belongs to the STXBP/unc-18/SEC1 family. STXBP1 may participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. It is essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. STXBP1 can interact with syntaxins 1, 2, and 3 but not syntaxin 4. It may play a role in determining the specificity of intracellular fusion reactions.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for STXBP1 antibody 67137-1-Ig | Download protocol |
| IHC protocol for STXBP1 antibody 67137-1-Ig | Download protocol |
| IF protocol for STXBP1 antibody 67137-1-Ig | Download protocol |
| FC protocol for STXBP1 antibody 67137-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
