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STXBP1 Polyclonal antibody

STXBP1 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 20562-1-AP

Print datasheet

Synonyms

EIEE4, MUNC18 1, N Sec1, p67, Protein unc 18 homolog 1, Protein unc 18 homolog A, RBSEC1, STXBP1, syntaxin binding protein 1, Unc 18A, UNC18, Unc18 1, UNC18A

验证数据展示

  • WB analysis using 20562-1-AP

    WB analysis using 20562-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20562-1-AP (STXBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 20562-1-AP

    IHC staining of human brain using 20562-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20562-1-AP (STXBP1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 20562-1-AP

    IHC staining of human brain using 20562-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20562-1-AP (STXBP1 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HeLa using 20562-1-AP

    IF Staining of HeLa using 20562-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using STXBP1 antibody (20562-1-AP) at dilution of 1:50 and CoraLite594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20562-1-AP targets STXBP1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name syntaxin binding protein 1
Synonyms EIEE4, MUNC18 1, N Sec1, p67, Protein unc 18 homolog 1, Protein unc 18 homolog A, RBSEC1, STXBP1, syntaxin binding protein 1, Unc 18A, UNC18, Unc18 1, UNC18A
Calculated Molecular Weight 68 kDa
Observed Molecular Weight68 kDa
GenBank Accession NumberNM_003165
Gene Symbol STXBP1
Gene ID (NCBI) 6812
RRIDAB_10694824
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP61764
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STXBP1, also named as UNC18A, N-Sec1 and p67 belongs to the STXBP/unc-18/SEC1 family. STXBP1 may participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. It is essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. STXBP1 can interact with syntaxins 1, 2, and 3 but not syntaxin 4. It may play a role in determining the specificity of intracellular fusion reactions. The antibody is specific to STXBP1.

实验方案

Product Specific Protocols
WB protocol for STXBP1 antibody 20562-1-APDownload protocol
IHC protocol for STXBP1 antibody 20562-1-APDownload protocol
IF protocol for STXBP1 antibody 20562-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols