验证数据展示
经过测试的应用
| Positive WB detected in | C6 cells, SH-SY5Y cells, U-251 cells, Jurkat cells, Neuro-2a cells, human brain tissue, rat brain tissue, mouse brain tissue |
| Positive IHC detected in | human brain tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | PC-12 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
60059-1-Ig targets Syntaxin 6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag1284 Product name: Recombinant human STX6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-255 aa of BC009944 Sequence: MSMEDPFFVVKGEVQKAVNTAQGLFQRWTELLQDPSTATREEIDWTTNELRNNLRSIEWDLEDLDETISIVEANPRKFNLDATELSIRKAFITSTRQVVRDMKDQMSTSSVQALAERKNRQALLGDSGSQNWSTGTTDKYGRLDRELQRANSHFIEEQQAQQQLIVEQQDEQLELVSGSIGVLKNMSQRIGGELEEQAVMLEDFSHELESTQSRLDNVMKKLAKVSHMTSDRRQWCAIAILFAVLLVVLILFLVL 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | syntaxin 6 |
| 别名 | 2B6G4, STX6, Syntaxin-6 |
| 计算分子量 | 29 kDa |
| 观测分子量 | 29 kDa |
| GenBank蛋白编号 | BC009944 |
| 基因名称 | Syntaxin 6 |
| Gene ID (NCBI) | 10228 |
| RRID | AB_2196504 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | O43752 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Syntaxin-6 (STX6) is a SNARE protein located in the trans-Golgi network (TGN) and endosomes. It associates with a variety of SNARE proteins and is involved in intracellular vesicle trafficking. STX6 is required for sorting proteins from endosomes toward either the TGN or lysosomes.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Syntaxin 6 antibody 60059-1-Ig | Download protocol |
| IHC protocol for Syntaxin 6 antibody 60059-1-Ig | Download protocol |
| WB protocol for Syntaxin 6 antibody 60059-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Suppression of ACE2 SUMOylation protects against SARS-CoV-2 infection through TOLLIP-mediated selective autophagy | ||
PLoS One Expression and functional characterization of His-tagged prestin in Chinese hamster ovary cells |
