• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of Y79 using 83298-1-RR (same clone as 83298-1-PBS)

    WB analysis of Y79 using 83298-1-RR (same clone as 83298-1-PBS)

    Y79 cells were subjected to SDS PAGE followed by western blot with 83298-1-RR (STX1B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83298-1-PBS in a different storage buffer formulation.

  • FC experiment of SH-SY5Y using 83298-1-RR (same clone as 83298-1-PBS)

    FC experiment of SH-SY5Y using 83298-1-RR (same clone as 83298-1-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Stx1b Recombinant Antibody (83298-1-RR, Clone:240156B9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83298-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00312-1

    Sandwich ELISA standard curve of MP00312-1

    Sandwich ELISA standard curve of MP00312-1, Human STX1B Monoclonal Matched Antibody Pair - PBS only. 83298-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag10275. 83298-4-PBS was HRP conjugated as the detection antibody. Range: 31.25-2000 pg/mL.

  • Sample test of MP00312-1

    Sample test of MP00312-1

    The mean human STX1B concentration was determined to be 7.2 μg/mL in rat Brain tissue lysate based on a 3.0 mg/mL extract load. The mean human STX1B concentration was determined to be 18.1 μg/mL in mouse Brain tissue lysate based on a 2 mg/mL extract load. The mean human STX1B concentration was determined to be 7.0 ng/mL in rat kidney tissue lysate based on a 3 mg/mL extract load. The mean human STX1B concentration was determined to be 15.1 ng/mL in mouse kidney tissue lysate based on a 1.5 mg/mL extract load.

  • IHC staining of mouse eye using 83298-1-RR (same clone as 83298-1-PBS)

    IHC staining of mouse eye using 83298-1-RR (same clone as 83298-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 83298-1-RR (STX1B antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83298-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Syntaxin 1A/1B antibody included
  • IHC staining of mouse eye using 83298-1-RR (same clone as 83298-1-PBS)

    IHC staining of mouse eye using 83298-1-RR (same clone as 83298-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 83298-1-RR (STX1B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83298-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Syntaxin 1A/1B antibody included
  • IHC staining of mouse brain using 83298-1-RR (same clone as 83298-1-PBS)

    IHC staining of mouse brain using 83298-1-RR (same clone as 83298-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83298-1-RR (STX1B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83298-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Syntaxin 1A/1B antibody included
  • IF Staining of mouse brain using 83298-1-RR (same clone as 83298-1-PBS)

    IF Staining of mouse brain using 83298-1-RR (same clone as 83298-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using STX1B antibody (83298-1-RR, Clone: 240156B9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83298-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 83298-1-RR (same clone as 83298-1-PBS)

    IF Staining of mouse brain using 83298-1-RR (same clone as 83298-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using STX1B antibody (83298-1-RR, Clone: 240156B9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83298-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00312-1

    Cytometric bead array standard curve of MP00312-1

    Cytometric bead array standard curve of MP00312-1, STX1B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83298-1-PBS. Detection antibody: 83298-4-PBS. Standard: Ag10275. Range: 1.25-40 ng/mL.

  • Affinity and Kinetic Characterization of 83298-1-RR

    Affinity and Kinetic Characterization of 83298-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83298-1-RR against Human STX1B were performed. The affinity constant is below 1 pM.

STX1B Recombinant antibody, PBS Only (Capture)

STX1B Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83298-1-PBS

产品说明书

CloneNo. 240156B9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

Syntaxin 1A, Syntaxin 1B, syntaxin 1B, STX1B2, STX1B1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy Syntaxin 1A/1B Ready-To-Use IHC Kit. Syntaxin 1A/1B primary antibody included.

产品信息

83298-1-PBS targets STX1B as part of a matched antibody pair:

MP00312-1: 83298-1-PBS capture and 83298-4-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse
免疫原 STX1B fusion protein Ag10275 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 syntaxin 1B
别名 Syntaxin 1A, Syntaxin 1B, syntaxin 1B, STX1B2, STX1B1
计算分子量 288 aa, 33 kDa
观测分子量 33-35 kDa
GenBank蛋白编号BC062298
基因名称 STX1B
Gene ID (NCBI) 112755
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP61266
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Syntaxins are a family of transmembrane proteins that belong to the SNARE complex (PMID: 11737951). In conjunction with other SNAREs and with the cytoplasmic NSF and SNAP proteins, syntaxins mediate vesicle fusion in diverse vesicular transport processes along the exocytic and endocytic pathways (PMID: 11737951). Syntaxin 1A and 1B, two closely related isoforms of syntaxin 1, are involved in synaptic vesicle docking and fusion during neurotransmitter release (PMID: 7690687; 1321498; 18691641).